We narrowed to 923 results for: Tro

1. Transferable Skills: Negotiation

   Type

   Blog Post

   ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

2. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

3. Hot Plasmids and Viral Preps - May 2021

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   ...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...

4. Transferable Skills Guide: Managing a Team

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   ...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   ...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

7. Plasmids 101: 5 factors to help you choose the right cloning method

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   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

8. 10 Basic tips for mammalian cell culture

   Type

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   ..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

9. Hot Plasmids: Winter 2025

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   ...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

10. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

11. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

12. Plasmids 101: Optimizing Plasmid Yields

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    ... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

13. Anatomy of a Plasmid Page at Addgene

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    ...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

14. SciComm: Taking Science to Elementary and Middle Schools

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    ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

15. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...  Susanna Stroik...

16. Course-Based Undergraduate Research Experiences (CUREs)

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    ... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

17. The Importance of a Fun Workplace: Company Culture at Addgene

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    ... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

18. A Career in Grants: How To Become a Grant Professional

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    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

19. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ...recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016...

20. New CRISPR Tools: Cas7-11 and PASTE

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    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...