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TypeBlog Post... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...
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Reflections and Looking Ahead
TypeBlog Post...find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes... -
Science Guides
TypeGuide...Pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the...Find educational guides to learn more about the different types of viruses that are commonly used in research... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog Post...never clicked for me. Recently my interest was renewed in part due to FOMO but mostly because of this.... And don’t forget to follow us @Addgene. References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...strives to match up scientists across fields with different technical knowledge that could help fight COVID... both clinical care and public health for this current COVID-19 pandemic, and outbreaks that have yet ... -
What's New in CRISPR - March 2020
TypeBlog Post...controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce...enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases for ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014...system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...meiosis in biology. Lots of it is researching the different aspects of the subject you're writing and then...developing new online courses about unique topics that aren’t already offered by eScience Labs. “We work with... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9... -
SunTag and Fluorescent Imaging
TypeBlog Post...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...includes an antibiotic resistance cassette and you aren’t introducing an additional antibiotic resistance...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science... -
Build Your CRISPR Vocabulary
TypeBlog Post...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects... -
10 Ways to Share Your Science!
TypeBlog Post...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...