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Showing: 821 - 840 of 1020 results
  1. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...
  2. Reflections and Looking Ahead

    Type
    Blog Post
    ...find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende...
  3. Science Guides

    Type
    Guide
    ...Pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the...Find educational guides to learn more about the different types of viruses that are commonly used in research...
  4. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because of this.... And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ...
  5. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete...
  6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...
  7. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...
  8. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...
  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...
  10. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ...
  11. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...
  12. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...
  13. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...
  14. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...
Showing: 821 - 840 of 1020 results