We narrowed to 916 results for: tro

1. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

2. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

3. Cloning Mammalian Cells with the Agarose Method

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   ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

4. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

5. 10 Basic tips for mammalian cell culture

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   ..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

6. Transferable Skills: Negotiation

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   ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

7. Plasmids 101: Optimizing Plasmid Yields

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   ... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

8. Anatomy of a Plasmid Page at Addgene

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   ...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

9. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...  Susanna Stroik...

10. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

11. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

12. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

13. A Career in Grants: How To Become a Grant Professional

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    Blog Post

    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

14. How to Be an Excellent Trainee

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    Blog Post

    ... Susanna Stroik...

15. Mesothelioma - Causes, Symptoms, and Treatment

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    ...recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016...

16. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ...SHERLOCK had a few limitations when it was first introduced in 2017 so the lab made further modifications...

17. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

18. Plasmids 101: Codon usage bias

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    Blog Post

    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...

19. To Codon Optimize or Not: That is the Question

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    Blog Post

    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...

20. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...