... other projects, using stem cells to develop in vitro models for lung disease. Darrell strives to promote...the interview with Darrell Kotton Chapter 1: Introduction to Darrell Kotton and the Boston University ...reliable projects. Chapter 9: Using stem cells as in-vitro models of disease. How Darrell is using stem cells...helping us stick to our core mission. Chapter 13: Outro - remember to step back and ask, "Why did I start...

...positive selection (ex: antibiotic selection and auxotrophy) and negative selection (ex: toxin-antitoxin ...technique from David Liu’s lab that gives more control and flexibility to CRISPR editing. Using a fusion...transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and all possible base-to-base... and Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic...

...any nascent technology, researchers are having trouble getting NgAgo genome editing up and running in ...get a better idea of how many people are having trouble, to determine if there have been any early successes... users likely need more time to optimize and troubleshoot the system. Site Directed Mutagenesis by PCR... with Enhancers and Epigenomics 5 Tips for Troubleshooting Viral Transductions Single Base Editing with...

...can easily spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never...could easily lend one to another labmate? Though electronic lab notebooks (ELNs) have gained traction with...to borrow most frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox...documents from anywhere, but also allow you to control who can edit the documents and track the revision...

...range from researching an area, to writing an introduction that gives a basic overview of kinematics in... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...traveling with the sales team to meet faculty and introducing the kits to clients who might initially be doubtful...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens....

...          Special Thanks Trophies and engraving services from Hit Trophy Additional Resources on the...positively affect scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed...distributed more than 100 times. We hope these desktop trophies will serve as badges of honor for our powerhouse...

... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...pharmacokinetics and biodistribution therefore introduce additional confounding factors when attempting...reporter genes for in vivo imaging Following in vitro characterization, Bourdeau et al performed a proof-of-concept...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...

... eSpCas9 and SpCas9-HF1. By mining the treasure trove of naturally occurring Cas9 diversity via the Integral... Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity or longevity. GeoCas9 is active...Besides being able to take some heat, GeoCas9 can introduce genomic edits in mammalian cells. Harrington et...GeoCas9’s thermostability could also be used with in vitro molecular bio applications that require high temperature...

...it is non-invasive and offers a high degree of control over time and space. Zebrafish have become a popular...are not native to vertebrates and are commonly introduced in zebrafish through the UAS/Gal4 system for ...had previously been a mystery, but through the control offered by the optogenetics toolbox and the versatility..., Filippo Del Bene, and Herwig Baier. "Optical control of zebrafish behavior with halorhodopsin." Proceedings...

...serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides sequences ...variable loops is one method for changing a capsid’s tropism.  cap also encodes the Assembly-Activating Protein...References: Naso MF, Tomkowicz B, Perry WL III, Strohl WR (2017) Adeno-Associated Virus (AAV) as a Vector...