We narrowed to 1,073 results for: TOR

...highly voted questions,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted...meant that the posters could be viewed publicly and stored in one place if the participants used the conference...

...persistent and exist online indefinitely. PIDs historically referred to objects that exist online, but this...to ensure that materials available through our repository are verified and identifiable to save researchers...

...PCR to generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, ...simple as mixing fragments together with linearized vector for seamless joining of multiple mutations spread...

...them. With these fantastic tools it’s now easy to monitor protein expression, localization, and protein-protein...Fatima’s development as a researcher and science communicator.  Update (7/17/2018): Check out Fatima's recent...

...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid ...

...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This Essential...Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development Plan Resources...

...confirm the presence of an insert in a particular vector by excising the insert from the backbone. To do...will need to know both the approximate size of the vector backbone as well as the predicted size of the insert...

...for your experiments, there are three important factors to consider: site accessibility, specificity, and...less likely to elicit a strong immune response, factors that may make it especially suitable for in vivo...

...interest is a low abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion...

...other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

...by screen readers to provide visually impaired visitors with information about images that a sighted reader...be, for example, a graduate student using viral vectors for the first time and learning how to purify AAV...

...is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and SLIC can...genome. REPLACR-mutagenesis starts out in familiar territory, but quickly diverges from previously discussed...

...expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature, ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...

..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...

...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

...fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, the red fluorescent...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

...with Beckman Coulter and that certainly played a factor in our decision to go with this machine, but we...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

...deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...cells with Nanoblades Primary cells are often refractory to gene delivery methods, but the researchers...