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We narrowed to 1,073 results for: TOR

Showing: 861 - 880 of 1073 results
  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...PCR to generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, ...simple as mixing fragments together with linearized vector for seamless joining of multiple mutations spread...
  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid ...
  3. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This Essential...Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development Plan Resources...
  4. Antibodies 101: ChIP

    Type
    Blog Post
    ...interest is a low abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion...
  5. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...
  6. Clear and Accessible Writing

    Type
    Blog Post
    ...by screen readers to provide visually impaired visitors with information about images that a sighted reader...be, for example, a graduate student using viral vectors for the first time and learning how to purify AAV...
  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...
  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...
  9. Summer SciComm: Preprints

    Type
    Blog Post
    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...
  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...
  11. Lab Automation at Addgene

    Type
    Blog Post
    ...with Beckman Coulter and that certainly played a factor in our decision to go with this machine, but we...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...
Showing: 861 - 880 of 1073 results