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  1. Improved Plasmid Maps Powered by SnapGene

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    Blog Post
    ...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  2. Hot Plasmids - May 2022

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    Blog Post
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

  3. Antibodies 101: ChIP

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    Blog Post
    ...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

  4. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

  5. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

  6. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post
    ...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post
    ...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania...

  8. Plasmid Tools for Microbiome Studies

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    Blog Post
    ...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering...

  10. Visualizing Protein Turnover In Situ

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    Blog Post
    ...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

  11. Addgene: The First Twenty Years

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    Blog Post
    ...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant...

  13. Developing Lab Management Software for Biology

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    Blog Post
    ...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what...

  15. Plasmids 101: Cre-lox

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    Blog Post
    ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

  19. Viral Vectors 101: Virus Safety

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    Blog Post
    ...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ...
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