We narrowed to 941 results for: Ina

1. Viral Vectors 101: Types of viruses

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   ...is not currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition...

2. 15 Years of Addgene: The Top 15 Plasmids

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   ...Note: this plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed...

3. Screening for Successful Genome Editing with Digital PCR

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   ... number of target DNA molecules present in the original sample with a great deal of precision. This means...

4. Plasmids 101: Methylation and Restriction Enzymes

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   ...as depicted in Lane 2. Controlling methylation Finally, you can control methylation by altering your choice...

5. Plasmids 101: Blue-white Screening

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   .... (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac...

6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two...

7. 3 Challenges in Plant Synthetic Biology

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   ... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...

8. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   ...energy and effort into making the startup self-sustainable. Within a year from this decision, Mike and his...

9. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...feedback for improvement. Go to a science or career seminar together and meet to debrief on the topic (career...

10. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ... you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships for...

11. Antibodies 101: Conjugation

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    ...Mueller BM, WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for...

12. Plasmid Tools for Microbiome Studies

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    ...of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins...

13. RaPID Detection of RNA-protein Interactions

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    ... RNA bait). Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome...

14. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...study epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety ...

15. RANbodies: Reporter Nanobody Fusions

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    ...antibodies are produced in animals instead of via recombinant DNA, they can’t be genetically tagged and instead...

16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    ...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization...

17. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...conformationally restricted RNA which improves mismatch discrimination and is more nuclease-resistant, resulting in...

18. Antibodies 101: Selecting the Right Antibody

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    ...could meet your needs.    Multiplex experiments Finally, consider whether you will be using other antibodies...

19. Transferable Skills Guide: Public Speaking

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    ...comfortably engage with you. If you can give a seminar, lead a journal club, or present at lab meetings...

20. Plasmids 101: The protein expression toolbox

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    ...excised by intentional introduction of the Cre recombinase. This is a time-tested system but does require...