We narrowed to 1,015 results for: NTS;

... proteins in living cells and organisms. Both components of the chimera are encoded by DNA. Since researchers...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One...

...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

...display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations compared...significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling prior to the...

...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...that isolates proteins from different cellular compartments such as the mitochondria, cytoplasm and nucleus...

...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561...

...digest appears normal and produces the expected fragments for that plasmid. This phenomenon indicates the...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...

...1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre recombinase...

...fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector ...you will find this information useful for your experiments. Don’t hesitate to check our lentiviral vector...

...more genes, protein coding sequences, or other elements inserted into it. You will have the option to ...plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is a Bacterial...

...CRISPR 101 eBook  Resources on the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant...2018). Real‐time observation of flexible domain movements in CRISPR–Cas9. The EMBO Journal, 37(10), e96941...

...find an introduction to viral vectors and their elements; resources on different types of vectors, capsids...

...describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...

...planning, executing, and troubleshooting your experiments. For example, if you’re very new to antibodies...

...thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like to give a shoutout to BosLab...

...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can we!...

... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

...Framed photos and slideshows shared some of the moments we captured over the years, including an album ...

...progress in all areas of research. We welcome your comments... Where do you think these technologies are going...

...ChiTownBio) and one of our favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together...

...to access helpful resources for their CRISPR experiments.  Many thanks to:  Daniel Mejías Sánchez, PhD...