We narrowed to 921 results for: Por

1. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential...

2. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

3. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   ...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ...

4. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   ... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective...

6. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   ...& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag...

7. DNA Purification Without a Kit

   Type

   Blog Post

   ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

8. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ... let us know! Contact our scientific technical support at [email protected] and let us help.  Check back...

9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   ... enough for efficient library performance, an important criterion for probing a limited number of cells...

10. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    ...The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy...

11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    ... Screens Before diving into the results, it’s important to note that in Morgens et al., guides were considered...

12. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...

13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and...

15. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

16. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

17. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    ...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

18. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

19. A Control for All Seasons

    Type

    Blog Post

    ...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...

20. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    ...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...