We narrowed to 931 results for: Por

1. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...production process? These questions are especially important when producing multiple vectors in parallel because...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

4. Viral Vectors 101: Producing Your rAAV

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   ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...

5. Antibodies 101: Flow Compensation

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   ...change your compensation values during the analysis portion of your experiment. So, as long as you are able...

6. Of Myc and Men

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   ... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

7. Plasmids 101: Optimizing Plasmid Yields

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   ... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...

8. Light Sheet Fluorescence Microscopy

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   ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

9. Tips for Using BLAST to Verify Plasmids

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   ...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential...

10. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective...

12. Viral Vectors 101: The Retroviral Lifecycle

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    ...& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag...

13. CRISPR 101: RNA Editing with Cas13

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    ...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ...

14. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post

    ...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

15. DNA Purification Without a Kit

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    ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

16. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ... enough for efficient library performance, an important criterion for probing a limited number of cells...

17. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ... let us know! Contact our scientific technical support at [email protected] and let us help.  Check back...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....

19. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post

    ...these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy...