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  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “...

  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....

  11. Viral Production at Addgene

    Type
    Blog Post
    ... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...

  13. Hot Plasmids Spring 2024

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    Blog Post
    ...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...FRET experiments. You may have to design several different constructs to find one that could be used for ...Fluorescent protein FRET: the good, the bad and the ugly. Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann...

  16. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

  17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Over 25 million people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be...for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski...

  18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example, in a two-guide...Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice...

  20. New Antibody Animation!

    Type
    Blog Post
    ...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...
Showing: 881 - 900 of 1011 results