We narrowed to 1,006 results for: RING
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TypeBlog Post...reproducibility. The Trimmer Lab found that in addition to recovering RNA from hybridomas grown in tissue culture, ...
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Sweating the Small Stuff: Details in the Lab
TypeBlog Post...double-checking details, or just taking a moment to relax. During really stressful days, I would even take advantage... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... alternative antimicrobial. Conjugates And HRP brings us handily to the final topic of this post, the... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...? CasPEDIA operates like many search engines, featuring both a general search bar tool and an advanced... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...brightness of two orange fluorescent proteins, differing by only a single amino acid. Without further information... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...would need to be generalizable and capable of measuring a variety of molecular signatures or cancer phenotypes... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...Cpf1 vs. Cas9, see the table below. Table 1. Comparing the Cas9 and Cpf1 CRISPR Nucleases Cas9 Cpf1... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...of NHEJ to generate translational reading frame-altering indels for “functional knockout” of a gene of ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...presented on this at the ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited... -
Chemogenetics Guide
TypeGuide...through the Gαq/11 G-protein and activate neuronal firing through stimulating phospholipase C, which releases...muscarinic G αq CNO* Increase Ca 2+ Neuronal burst firing Armbruster et al., 2007 rM3D Rat M3 muscarinic ...adrenergic G αs CNO* Increase cAMP Neuronal burst firing Guettier et al., 2009 hM4Di hM2Di Human M4 muscarinic...and Sternson SM (2011). Chemical and genetic engineering of selective ion channel-ligand interactions....Moore CI, Shaner NC, Hochgeschwender U (2024). Engineering luminopsins with improved coupling efficiencies...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,... -
RNA Extraction Without A Kit
TypeBlog Post...further streamline this process and/or stabilize RNA during tissue collection and homogenization. The most ... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...transferable skills while working in the lab and by offering advice on areas where you might need some help... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...protein degradation Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...shouldn’t work for free.” Susan also suggests volunteering to write for a non-profit, like she did for ... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein...