We narrowed to 1,010 results for: RING
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TypeBlog Post...of NHEJ to generate translational reading frame-altering indels for “functional knockout” of a gene of ...
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Top Requested Lentivirus and AAV of 2016
TypeBlog Post...2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post... Addgene, we now use next-generation sequencing during our QC process. This way, we can confirm the sequence... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...reproducibility. The Trimmer Lab found that in addition to recovering RNA from hybridomas grown in tissue culture, ... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...double-checking details, or just taking a moment to relax. During really stressful days, I would even take advantage... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... alternative antimicrobial. Conjugates And HRP brings us handily to the final topic of this post, the... -
Viral Vectors 101: Viral Applications
TypeBlog Post...host cell (the target of viral infection) and delivering a genetic payload. This cargo is typically their... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...? CasPEDIA operates like many search engines, featuring both a general search bar tool and an advanced... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...materials in our catalog are very well-characterized. During this time, we’ve also created a variety of educational... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...presented on this at the ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...protein degradation Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...shouldn’t work for free.” Susan also suggests volunteering to write for a non-profit, like she did for ... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...transferable skills while working in the lab and by offering advice on areas where you might need some help... -
RNA Extraction Without A Kit
TypeBlog Post...further streamline this process and/or stabilize RNA during tissue collection and homogenization. The most ... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein... -
Developing Lab Management Software for Biology
TypeBlog Post...simple task in the lab, but the software should be mirroring the lab procedures, not artificially constraining... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... Mary Gearing... -
The Future of Research Symposium Boston 2015
TypeBlog Post...participation in this panel, Professor Stephan will be delivering the keynote address at the symposium. Eve Marder...