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We narrowed to 1,052 results for: STI;

Showing: 881 - 900 of 1052 results
  1. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...advice for using Twitter, this post provides suggestions for using LinkedIn and Facebook. For each of ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...
  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...
  3. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...
  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...
  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...
  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...
  7. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...
  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...
  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...
  10. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    .... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...
  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...
  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated...
  13. Hot Plasmids - October 2020

    Type
    Blog Post
    ...six different genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet...the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half life of 84 hours in...
Showing: 881 - 900 of 1052 results