We narrowed to 1,045 results for: STI;

... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...

...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

...sensory input from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory... demonstrate pain response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration...

...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

... in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, we used primary...the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins...

.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

...proteins interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with...

...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and a mutated version of the ...

...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...

... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated...

...six different genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet...the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half life of 84 hours in...

...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... edit. If possible, consider following these suggestions: Edit the PAM along with your intended edit....

...sample protein is retained better, antibodies will stick permanently to a membrane that has been dried, no...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...

...modifying enzyme) to pair with it can be queried by testing any number in the Level 2 assembly. These pathways...scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, synthetic ...

...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice...

...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

...used to expand already-existing sets. Note that this tool relies on a stochastic search algorithm rather...

...enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently, FRET...