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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,044 results for: STI;

Showing: 881 - 900 of 1044 results

  1. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...

  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    .... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

  5. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

  8. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

  9. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...

  12. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...impacts the expression level of the transgene. In existing strains of mice, the transgene can be maintained...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...

  13. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...six different genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet...the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half life of 84 hours in...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    ...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... edit. If possible, consider following these suggestions: Edit the PAM along with your intended edit....

  17. Hot Plasmids February 2024

    Type
    Blog Post
    ...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...sample protein is retained better, antibodies will stick permanently to a membrane that has been dried, no...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...
Showing: 881 - 900 of 1044 results