We narrowed to 1,082 results for: TOR

...expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature, ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...

..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...

...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

...fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, the red fluorescent...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

...with Beckman Coulter and that certainly played a factor in our decision to go with this machine, but we...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

...deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...cells with Nanoblades Primary cells are often refractory to gene delivery methods, but the researchers...

...., Langhorst, B. W., Bilotti, K., Pryor, J. M., Cantor, E. J., Canton, B., Knight, T. F., Thomas C. Evans...J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot...

...? In this post we will review some of the main factors that affect isotype choice. Target and application...when choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety...

...the experiments performed by our thousands of depositors. That means their original articles are your ...searches scientific articles, patents, and even some doctoral dissertations. Streamline the question asking...

...a method  As with all experiments, a number of factors will determine which approach is right for your...number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791...

...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

...deliver in low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al....

...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

...exclusive. In the first, the replication initiation factor RepA binds to the iteron to initiation replication... than simply competing for replication control factors and tends to be more of a concern with low copy...

...In mathematics, I think this is represented by factorials. In environmental science and ecology, this can... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...another job, it will be important to retain the stories and examples that have guided your resolution processes...

... RNA pool was cloned into bacterial expression vectors to create a library. Bacteria were transformed,...Jaffrey group was able to quantify the effect of mTOR inhibition on the activity of RNA Polymerase III...