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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,031 results for: ell

Showing: 881 - 900 of 1031 results

  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

  3. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...

  4. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...

  5. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...

  7. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

  10. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook...

  18. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive...

  19. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...hard-to-read sentences.  Grammarly Grammarly is a lot like spell check, but since it’s an internet browser plug-in...managers and productivity tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront...
Showing: 881 - 900 of 1031 results