We narrowed to 1,046 results for: fer

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...and industry jobs. Since there are a variety of different career paths that don’t all need another degree...-op. It has been so insightful to learn about different career paths, successes and failures, values, ...

2. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   ... to address the unique needs of students from different departments across the university and alterations...public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical...

3. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ...academic labs. Being unable to publish or attend conferences makes it very challenging to compete in today...pressure to publish and more freedom to attend conferences, but after receiving her degree she was faced...

4. Academic vs. Industry Postdocs

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   ... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

5. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...treatment (Stephens et al., 2019). If sufficient gene transfer and knock-in is achieved, producer cells could...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...

6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

7. 3D Printing Meets CRISPR Cas9

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   ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

8. Plasmids 101: Knockout/Knock-In Plasmids

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   ...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

10. SciComm: Taking Science to Elementary and Middle Schools

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    ...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

11. Summer SciComm Series: Modes of Communication

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    ...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

13. CRISPR Antimicrobials

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    ...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

14. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...

15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...Red-shifts the wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes identified...Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement...

17. The Fluorescent Vegetables in Aptamer Soup

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    ...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

18. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...existing comprehensive mutagenesis methods, PFunkel offers the best combination of library coverage, mutational...mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis...

19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ...recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering...more at his webpage or follow him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman...

20. New Antibody Animation!

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    ...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...