Hot Plasmids and Viral Preps - May 2021
Type
Blog Post
... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...
We narrowed to 931 results for: por
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Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...production process? These questions are especially important when producing multiple vectors in parallel because... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...community, will soon expand to include several other important pathways. We invite feedback and contributions... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...Researchers from a variety of institutions recently reported their inability to recapitulate the results of... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter... -
DNA Purification Without a Kit
TypeBlog Post...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w Zou... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post... let us know! Contact our scientific technical support at [email protected] and let us help. Check back... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post... enough for efficient library performance, an important criterion for probing a limited number of cells... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...Hu et al., 2018). In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants.... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy...