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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 451 - 460 of 1010 results

  1. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost... red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...you can see in Figure 1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps...hypothetical experiment in the lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured...

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...in order to directly show that, for example, the firing of a specific type of neuron in a specific brain...whether you wish to stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the... which results in depolarization and subsequent firing of an action potential. Halorhodopsins (HR) are...may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery method...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  3. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an... experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable...can search by any part of the data. For example, entering ‘C. elegans’, or ‘elegans’ will filter the table...CRISPR Technologies Available Through Resource Sharing. 2016. In press. Additional Resources on the Addgene...

  4. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...unmatched customer support, as this is vital to ensuring that our services help accelerate your research...support team that can help you through any search, ordering, technical, or quality control issues. Improving...within the widget, you’ll navigate to articles answering your questions making it easier than ever to get...describe your issue and press send. Doing so will bring your concern directly to our support team’s attention...

  5. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ..., Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating...normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing...and relevance for the efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva... Mary Gearing...

  6. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...success.” 2. “Enjoy Teamwork.” Be friendly, open, sharing, rather than intensely competitive. Be curious ...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...connected to scientists and use the skills they learned during their studies. They suggest that you make time ...Connie Cepko, Tom Ellis, and George Church for answering our burning questions about how they manage and...

  7. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...can remain present until cleared by the body. Administering the drug once can result in several hours of... Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to...injection. The main challenge in using ligands is ensuring the agonist is able to cross the blood brain barrier...Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...hydrogen bonds, destabilizing the molecule and favoring the separation of the two helixes. Low pH instead...chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially...especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple...

  9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed your experiment using...sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may...negative selection strategies adopt naturally occurring genes for cloning purposes (antibiotic resistance... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...

  10. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to stabilize...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture...reducing the read depth necessary when sequencing and lowering the amount of tissue needed per experiment. In...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model...
Showing: 451 - 460 of 1010 results