We narrowed to 941 results for: Ina

1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization...

2. Transferable Skills Guide: Public Speaking

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   Blog Post

   ...comfortably engage with you. If you can give a seminar, lead a journal club, or present at lab meetings...

3. Plasmids 101: The protein expression toolbox

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   ...excised by intentional introduction of the Cre recombinase. This is a time-tested system but does require...

4. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ... going forward. It’s just not that easy. All combinations of donor/recipient interaction should be tested...

5. Antibodies 101: Conjugation

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   ...Mueller BM, WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for...

6. All in a Twist: dsRNA

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   Blog Post

   ... allowing the DNA strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle...

7. The Future of Research Symposium Boston 2015

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   Blog Post

   ...to discuss their visions for a successful and sustainable biomedical research sector at our first Future...

8. Working with Nuclear Receptors

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   ...) McKenna, N. J.and O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors...

9. 5 Steps to Writing Grant Proposals for Grad Students

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   Blog Post

   ... technologies! If data from a publication or preliminary data suggests the feasibility of the statement...

10. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...other ways to make your poster more accessible. Final thoughts Though we’ve just presented some standard...

11. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...proof-of-principle, the team used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in...

12. Open Resources and Plasmid Tools For Studying C. elegans

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    ...microscopy and computer imaging processing. The combination of these methods provides unique opportunities...

13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...downstream of the transcription start site will terminate transcription of most of the ncRNA (Figure 1D)...

14. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...strength of bands from different rounds of detection. Finally, stripping and reprobing works best with western...

15. Progress Towards a PAM-Free CRISPR

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    ...are not in your favor. Thus, there is a need to eliminate or reduce the PAM requirement to allow flexibility...

16. Viral Vectors 101: Calcium Sensors

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    ...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

17. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...the background staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find...

18. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications...

19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post

    ... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...of only the broadest-targeting variants. For the final round of evolution, they used a no-G PAM library...