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We narrowed to 1,135 results for: APP

Showing: 901 - 920 of 1135 results
  1. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA ...reading results from a NanoDrop. The principles will apply to similar instruments from other companies, like...sample will have minimal impact on downstream applications, but they can impair your ability to get an ...be sure it is compatible with your downstream applications and that you use the same solution for blanking...are, of course, more considerations and other approaches for measuring DNA quantity and quality than we...A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression Newsletter. Issue 15...
  2. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...these proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...), which is especially valuable for regulatory approval and public acceptance. Recently, viral vectors... is where TnpB offers a clear advantage. At approximately one-third the size of Cas9 or Cas12a, TnpB is...viral vectors. Even for advanced genome editing approaches like base editing and prime editing (Molla et...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...
  3. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ...This distinct approach means researchers can now select the best tool for their application from among many...incorporate A bases across from abasic sites. This approach resulted in a maximum A → T product purity of ...AYBEv3!). Although AYBE, AXBE, and ACBE do not approach the high product purity of CBEs and ABEs, they...). Several different teams succeeded with this approach around the same time (He et al., 2024; Tong et...on in the base editing field! From CRISPR-free approaches to mitochondrial base editing to base editors...
  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...performs well in many different antibody-based applications.  IPI’s recombinant version is a fusion of mouse...ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders. But CRBN.... elegans embryo, where developmental processes happen within a few hours. In order to use the latest ...cellular processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our...when the module unfolds under tension. When this happens, BiT and the LgBiT luciferase fragment (expressed...
  5. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post
    ...difficult to source a commercially available and appropriately conjugated antibody. While labs can conjugate...find,  it is often easier for labs to source appropriate antibodies for an indirect ELISA than for a direct... capture antibody must bind to distinct, non-overlapping epitopes so that they do not interfere with each...specific and can be difficult to develop if an appropriate conjugated antibody is not readily available....cross-reactivityChallenging to develop assay Competitive Appropriate for small moleculesReduced chance of cross-reactivity...choose the best ELISA for your needs. Good luck and happy binding! More resources on the Addgene blog Antibodies...
Showing: 901 - 920 of 1135 results