We narrowed to 1,135 results for: APP

...on counting the number of autophagosomes. This approach, however, has inherent limitations because it ...

...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature...

...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...

...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...

... and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA ...reading results from a NanoDrop. The principles will apply to similar instruments from other companies, like...sample will have minimal impact on downstream applications, but they can impair your ability to get an ...be sure it is compatible with your downstream applications and that you use the same solution for blanking...are, of course, more considerations and other approaches for measuring DNA quantity and quality than we...A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression Newsletter. Issue 15...

...these proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...), which is especially valuable for regulatory approval and public acceptance. Recently, viral vectors... is where TnpB offers a clear advantage. At approximately one-third the size of Cas9 or Cas12a, TnpB is...viral vectors. Even for advanced genome editing approaches like base editing and prime editing (Molla et...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...

...This distinct approach means researchers can now select the best tool for their application from among many...incorporate A bases across from abasic sites. This approach resulted in a maximum A → T product purity of ...AYBEv3!). Although AYBE, AXBE, and ACBE do not approach the high product purity of CBEs and ABEs, they...). Several different teams succeeded with this approach around the same time (He et al., 2024; Tong et...on in the base editing field! From CRISPR-free approaches to mitochondrial base editing to base editors...

...performs well in many different antibody-based applications.  IPI’s recombinant version is a fusion of mouse...ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders. But CRBN.... elegans embryo, where developmental processes happen within a few hours. In order to use the latest ...cellular processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our...when the module unfolds under tension. When this happens, BiT and the LgBiT luciferase fragment (expressed...

...difficult to source a commercially available and appropriately conjugated antibody. While labs can conjugate...find,  it is often easier for labs to source appropriate antibodies for an indirect ELISA than for a direct... capture antibody must bind to distinct, non-overlapping epitopes so that they do not interfere with each...specific and can be difficult to develop if an appropriate conjugated antibody is not readily available....cross-reactivityChallenging to develop assay Competitive Appropriate for small moleculesReduced chance of cross-reactivity...choose the best ELISA for your needs. Good luck and happy binding! More resources on the Addgene blog Antibodies...

...The LipoGlo system represents a new, intriguing approach to expand our understanding of ApoB-LPs and accelerate...

...ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory of DREADDs / Chemogenetics...

...has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition...

...you choose could be based on your downstream application, time, and instrument availability. UV absorbance...

... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...

...mix chemicals together in chemistry to see what happens, but there isn’t really an equivalent of a “chemistry...

...the biotech revolution. Our lab exists in what appears to be a funky old garage, but looks can be deceiving...

...by TUNEL staining.  The video below shows what happens to the zebrafish’s heartbeat and blood circulation...

...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...

... an upright position for 30 seconds, and then recapping the tubes prior to thaw. We compared vented and...

... we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for...