We narrowed to 960 results for: App

1. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   ...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...MiSeq and set up the run making sure to note the appropriate barcode for each sample. About 24 hours later...

3. Hot Plasmids and Viral Preps - March 2021

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   ...from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody pairs. Because the...

4. Illuminating Epigenetics with A FRET Based Biosensor

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   ...risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can...

5. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...institutions are not local but are national issues. I applied and was successfully granted a travel award to ...

6. Experimenting in an Open Source lab: from CRISPR to Cats

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   ... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics software to...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you...

9. Common Injection Routes in Mice

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   Blog Post

   ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

10. Transferable Skills Guide: Managing a Team

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    Blog Post

    ... couple of weeks now”. I had no idea this was happening even though I met with X on a weekly basis! The...

11. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...those flames about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication...

12. Genome-wide Screening Using CRISPR

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    Blog Post

    ...negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell...

13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

14. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits...

15. CRISPR 101: RNA Editing with Cas13

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    ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

16. Negative Can Be Positive: Open AAV Data with Addgene

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    ...this information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV...

17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents...

18. DNA Purification Without a Kit

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    Blog Post

    ...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

19. Plasmids 101: Protein tags

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    Blog Post

    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

20. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...