We narrowed to 916 results for: IRE;

1. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

   Type

   Protocol

   ...wear glasses/goggles in addition to the BSL-1 requirements. Conclusion Although simple, following appropriate...

2. Protocol - Over-Agar Antibiotic Plating

   Type

   Protocol

   ... in LB medium. The concentration of antibiotic required for effective over-agar selection has been empirically...

3. Weighing Reagents Protocol

   Type

   Protocol

   ...balance and then tare it. Then add the material directly to the tube to weigh it. Make sure that your scoopula...

4. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ...linearize a plasmid to determine the size of the entire construct or excise some or all of an insert from...

5. Ligation Independent Cloning

   Type

   Protocol

   ...The annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for...

6. Fluorescence Titering Assay

   Type

   Protocol

   ...the cells in the remaining well, a cell count is required for calculating the titer. Incubate for 48–72 ...

7. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ... ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction volume usually...

8. General Transfection

   Type

   Protocol

   ...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

9. Protocol - Bacterial Transformation

   Type

   Protocol

   ... μl of a 1:5 or 1:10 dilution rather than 1 μl directly....

10. Gibson Assembly Protocol

    Type

    Protocol

    ...and yield. If there are significant amounts of undesired product, gel-purify DNA segments. Otherwise, PCR...

11. Lentivirus Production

    Type

    Protocol

    ...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

12. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ... positively charged and will run the opposite direction from the DNA. So if you run the gel without EtBr...

13. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...antibiotic in culture, or remove the antibiotic entirely. If the antibiotic is reduced or removed from ...

14. Coomassie Purity Stain of Recombinant Antibodies

    Type

    Protocol

    ...Figure 2 Using the box tool, draw a box around the entire first gel lane (as in Figure 2). Select Analyze...

15. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...which should be on the same focal plane. Scan the entire plate and make note of each well in which you see...

16. Lentivirus ddPCR Titration

    Type

    Protocol

    ...droplets are not disrupted, insert the pipette tips directly in the center of the well and tilt to a 45° angle...