We narrowed to 912 results for: IRE;

...The annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for...

...the cells in the remaining well, a cell count is required for calculating the titer. Incubate for 48–72 ...

...and yield. If there are significant amounts of undesired product, gel-purify DNA segments. Otherwise, PCR...

... ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction volume usually...

...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

... μl of a 1:5 or 1:10 dilution rather than 1 μl directly....

...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

... positively charged and will run the opposite direction from the DNA. So if you run the gel without EtBr...

...antibiotic in culture, or remove the antibiotic entirely. If the antibiotic is reduced or removed from ...

...Figure 2 Using the box tool, draw a box around the entire first gel lane (as in Figure 2). Select Analyze...

...which should be on the same focal plane. Scan the entire plate and make note of each well in which you see...

...droplets are not disrupted, insert the pipette tips directly in the center of the well and tilt to a 45° angle...