We narrowed to 929 results for: Ina

1. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   ...microscopy and computer imaging processing. The combination of these methods provides unique opportunities...

2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   Blog Post

   ...downstream of the transcription start site will terminate transcription of most of the ncRNA (Figure 1D)...

3. Early Career Researcher Toolbox: Finding Relevant Papers

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   Blog Post

   ...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications...

4. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ...strength of bands from different rounds of detection. Finally, stripping and reprobing works best with western...

5. Progress Towards a PAM-Free CRISPR

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   ...are not in your favor. Thus, there is a need to eliminate or reduce the PAM requirement to allow flexibility...

6. Viral Vectors 101: Calcium Sensors

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   ...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

7. Antibodies 101: Choosing the Right Isotype

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   Blog Post

   ...the background staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find...

8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   ... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...

9. Magnetic Control of Proteins: More than a Dream

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   ...possibility that this might just be an artifact and eliminate sources of interference, but the result persisted...

10. Transferable Skills: Negotiation

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    ...Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate with...

11. Antibodies 101: Flow Compensation

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    Blog Post

    ... you should be able to run your experiment and finalize your compensation values later. This can be helpful...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...

13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...of only the broadest-targeting variants. For the final round of evolution, they used a no-G PAM library...

14. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...contain two dimerizing FPs can exist in an open inactive state, a closed active state, and an artificially...

15. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...transduction or injection as either protein, RNA, or DNA. Finally, once you have identified the best expression system...

16. Which Fluorescent Protein Should I Use?

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    ...GFP also emits yellow light). Note that some combinations of FPs can cause an effect called FRET (fluorescence...

17. A Guide to Starting Your Own Journal Club

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    ...polls and surveys. A mix of closed (multiple choice/binary) and open questions is helpful to ensure acquisition...

18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...colleagues at NYU and visiting scientists, such as seminar speakers. They made an every effort to interact...

19. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...new directions, while the second approach could terminate with the rejection of a model and no clear alternatives...

20. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...for simple transfection experiments. Reporters Finally, in some cases a researcher may choose not impose...