All in a Twist: dsRNA
Type
Blog Post
... allowing the DNA strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle...
We narrowed to 941 results for: Ina
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What's New in CRISPR - November 2021
TypeBlog Post... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post.... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post... going forward. It’s just not that easy. All combinations of donor/recipient interaction should be tested... -
The Future of Research Symposium Boston 2015
TypeBlog Post...to discuss their visions for a successful and sustainable biomedical research sector at our first Future... -
Working with Nuclear Receptors
TypeBlog Post...) McKenna, N. J.and O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post... technologies! If data from a publication or preliminary data suggests the feasibility of the statement... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...downstream of the transcription start site will terminate transcription of most of the ncRNA (Figure 1D)... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...other ways to make your poster more accessible. Final thoughts Though we’ve just presented some standard... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...proof-of-principle, the team used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...microscopy and computer imaging processing. The combination of these methods provides unique opportunities... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...strength of bands from different rounds of detection. Finally, stripping and reprobing works best with western... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...the background staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...are not in your favor. Thus, there is a need to eliminate or reduce the PAM requirement to allow flexibility... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...of only the broadest-targeting variants. For the final round of evolution, they used a no-G PAM library...