We narrowed to 928 results for: Por

1. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...

2. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

3. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   ...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

5. A Control for All Seasons

   Type

   Blog Post

   ...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...

6. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   ...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...

7. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   ...to probing with cells in order to optimize this portion of the assay before attempting it with a random...

8. Antibodies 101: Antibody Engineering and Directed Evolution

   Type

   Blog Post

   ...means improving research. More specific antibodies support common research tools like western blots, ELISAs...

9. Deep Dive: Statistical Tests (Comparisons)

   Type

   Blog Post

   ...most effective and accurate way possible it’s important to select the right test for the job (and, uh,...

10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    ...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective...

11. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    ...target protein: instead, it recognizes a small portion of the protein, known as an epitope. Epitopes are...

12. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    ...Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing CRISPR 101: Cas9 Structural...

13. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    ...be entirely verified by sequencing. Upload any support files that will make it easier for scientists to...

14. DNA Quantification

    Type

    Protocol

    ... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...

15. Protocol - How to Design Primers

    Type

    Protocol

    ...toward one another. The size of the primer is very important as well. Short primers are mainly used for amplifying...

16. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type

    Protocol

    ...the safest way possible. Protecting yourself is important! Disclaimer: The SOP presented here has been designed...

17. Protocol - How to Streak a Plate

    Type

    Protocol

    ...initials. Labeling within a laboratory setting is important for organization, and it is recommended that you...

18. Weighing Reagents Protocol

    Type

    Protocol

    ...Conclusion Appropriately weighing reagents is important to ensure the accurate composition of your solutions...

19. Protocol - How to Inoculate a Bacterial Culture

    Type

    Protocol

    ...plasmid. However, a liquid culture is capable of supporting a higher density of bacteria and is used to grow...

20. Ligation Independent Cloning

    Type

    Protocol

    ...provided by your polymerase manufacturer. It is very important to remove all free nucleotides from your PCR product...