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We narrowed to 982 results for: Ran;

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Showing: 901 - 920 of 982 results

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ... is based on an LV titer range of 1E+05–1E+09 TU/mL, where TU is transducing units and generally represents...July 7, 2023 Workflow Timeline Day 1: Seed and transduce cells Day 4: Treat cells with Benzonase and harvest...Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare the following... pipette 10–20 times. Use a new pipette tip to transfer 200 µL of the first dilution into the next dilution... plate (1 dilution per well). Leave one well untransduced (add 150 µL of DMEM complete instead of a viral... into the wells containing the virus and the untransduced control. Pro-Tip For even seeding, prepare a...min. Resuspend cells in 500 µL DMEM complete and transfer to a microcentrifuge tube. Centrifuge for 5 min...

  10. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...step heats the double stranded DNA template strand to the point where the strands start denaturing and ...this temperature range to anneal to each of the single stranded DNA template strands. The DNA polymerase...of the sense and anti-sense strands of the template DNA. They are strands of nucleic acid that are starting...molar ratio. It also requires two unique single stranded DNA oligonucleotide (oligo) primers, which anneal...seconds at 94°C: Continued denaturation of double stranded DNA. Anneal primers for 30 seconds at 55°C: The...polymerase to synthesize and elongate the new target DNA strand accurately and rapidly. Repeat steps 2-4 25-30 ...fill-in any protruding ends of the newly synthesized strands. Materials List Reagents for each 50µL PCR: Thin-walled...

  11. CRISPR Library Amplification

    Type
    Protocol
    ... Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation should be...of 5 mL (3 mL SOC + 2 mL transformed Endura from two separate transformations). Pro-Tip Do not pipette...assumes familiarity with standard bacterial transformation and basic knowledge of the library being amplified...amplified. Please see our protocol on Bacterial Transformation . The generalized pooled library amplification...premixed LB (VWR 101414-072) for convenience but any brand or house- made LB that supports normal growth is...using a new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes...repeatedly or mix when removing SOC containing transformed DNA-Endura from cuvette. Shake four 14 mL Vented...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...incubation, transform DH5α E. coli cells by heatshock with the plasmid of interest. See our transformation page...for a detailed heatshock transformation protocol. Plate 50 µL of transformed E. coli /rescue media suspension...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...plating antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of antibiotic makes...investigator to conveniently plate and select transformed cells containing plasmids differing in their ... sterile water (or other antibiotic) E. coli transformed with a plasmid containing the carbenicillin (...carbenicillin, plated over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on ...

  15. A Look at Addgene's QC Process

    Type
    Blog Post
    ...accuracy across all of our plasmids and provide transparency in sequence discrepancies. While Addgene’s QC...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    ...probably difficult for you to ascertain where on this range your question falls. Here are some things to keep...
Showing: 901 - 920 of 982 results