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TypeBlog Post...Industry Professionals”. This larger shop might appeal to some although most scientist coaches can help...
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Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...influence (non-homologous end joining, or NHEJ), in approximately one percent of cases, the stem cell will seek... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post... also used to teach students about restriction mapping and western blotting. Students who participate ... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...MiSeq and set up the run making sure to note the appropriate barcode for each sample. About 24 hours later... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...from Tang et al., 2020. G-baToN can also be applied to other fluorophore/antibody pairs. Because the... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...choose essentially any background, but two common approaches are either a solid color, or a “themed” background... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...institutions are not local but are national issues. I applied and was successfully granted a travel award to ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives... -
Common Injection Routes in Mice
TypeBlog Post...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics software to... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you... -
Transferable Skills Guide: Managing a Team
TypeBlog Post... couple of weeks now”. I had no idea this was happening even though I met with X on a weekly basis! The... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...those flames about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication... -
Genome-wide Screening Using CRISPR
TypeBlog Post...negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits... -
DNA Purification Without a Kit
TypeBlog Post...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...