We narrowed to 951 results for: cha

1. CasPEDIA: A Functional Classification of Cas Enzymes

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   ...Institute. What is CasPEDIA? CasPEDIA is an online, searchable resource that provides summary information on...

2. Plasmids 101: Methylation and Restriction Enzymes

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   ...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

3. Plasmids 101: Blue-white Screening

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   ...pUC19 Let’s begin at the beginning. The well-characterized bacterial lac operon contains a gene called ...

4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...different biological systems. Although Xie et. al characterized the system in plants, PTGs will likely work ...

5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...

6. How to Make Friends and Meet People at a Scientific Conference

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   ... can also see and offer congratulations on job changes and promotions.  Follow academic scientists on ...

7. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ... Michael G. Lemieux...

8. Plasmid Tools for Microbiome Studies

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   ...29608282. PubMed Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad...

9. Viral Vectors 101: An Introduction to AAV

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   ...26814968. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

10. To Each HIS Own

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    ...small size of the polyhistidine tag minimizes the chance of the tag disrupting normal protein function, ...

11. Transferable Skills Guide: Cross-team Communication

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    ...In 2016, I was put in charge of the biggest project I’d seen in my already long tenure at Addgene. I ...

12. Antibodies 101: Secondary Antibodies

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    ...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

13. Summer SciComm Series: Cognitive Load

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    ...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

14. Troubleshooting Your Plasmid Cloning Experiment

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    ...the previous reactions. This will increase your chances to have a successful and ultimately faster cloning...

15. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ... On the other hand, slow growth can also cause changes in plasmid copy number. During the shift from exponential...

16. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...promotes gender equality in careers through structural change in research organizations. “Fix the Knowledge” ...

17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ...has been successfully applied to functionally characterize combinatorial gene knockouts generated via multiplexed...

18. New Tool for Lineage Tracing: The ClonTracer Library

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    ...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

19. Rabies and Neuronal Tracing

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    ...Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: ...

20. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...