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...more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang...

...2020). The generated ABE8s needed to produce 3 concurrent base edits to withstand antibiotic selection....great addition to the base editing toolbox.  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran...

... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection...

...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used...

...science. It's open-source. You can see all the current code on GitHub, ask us questions about it, or send.... It builds on existing popular web services, currently Crispor and Crispresso, that adhere to the same...

...will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “...

... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic...

... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent...

...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic...

...decorative" title="Check icon" %}   References and Resources References Hwang, J. M., Piccinini, T. E., Lammel...

...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....

...about science whenever he's not off the grid.   References Bischof J, Duffraisse M, Furger E, Ajuria L, ...plasmids and resources Visit our Molecular Biology Reference ...

...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...

... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson...

...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...

...address a diverse set of biological inquiries. References Ogura, Teru, and Sota Hiraga. "Mini-F plasmid... Addgene.org Check out our molecular biology reference Search for a plasmid here Find plasmids from our...

...FRET experiments. You may have to design several different constructs to find one that could be used for ...Fluorescent protein FRET: the good, the bad and the ugly. Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann...

...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

...labelling techniques. These newer tools are also currently available at Addgene.  BioID2: A smaller improved...protein or RNA motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity...