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Showing: 921 - 940 of 1086 results
  1. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...
  2. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...
  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that...
  4. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...while still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered...
  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-...
  6. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...
  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...calling this “physical distancing” since it’s important to stay virtually connected with the people in...webinars. Share your work online. Maybe you’ve always wanted to start using social media professionally but ...
  8. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...his love for science and later realizing that he wanted to continue doing research, Doench went on to apply...regulatory process. This early research is still relevant to his work at the Broad Institute today, where...
  9. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...
  10. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...
  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...
  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...for your audience. I ask myself that question constantly (and so should you!)  Amount of information and... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...
  13. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship...
Showing: 921 - 940 of 1086 results