We narrowed to 1,086 results for: ANT;
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TypeBlog Post...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly! The question The first, and most important question, you should ask for any science communication...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...as a drug-screening platform to identify new antifungals that target calcineurin. Current calcineurin ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire... -
Another Pathway into Cells: iTOP
TypeBlog Post... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...to publication and acquisition of funding. If a grant committee is not convinced that your project is ...getting a much-needed break from your lab bench. Want further information about shaping your career to... -
Pushing the Limits of DNA Assembly
TypeBlog Post...while still creating scarless plasmid inserts. Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used... -
25 Work From Home Tips for Scientists
TypeBlog Post...calling this “physical distancing” since it’s important to stay virtually connected with the people in...webinars. Share your work online. Maybe you’ve always wanted to start using social media professionally but ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...his love for science and later realizing that he wanted to continue doing research, Doench went on to apply...regulatory process. This early research is still relevant to his work at the Broad Institute today, where... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...circular DNA takes on several conformations the most abundant being: supercoiled, relaxed and nicked. If your...your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA will make it difficult... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post... genome-wide screening,” Konermann said. “If you want to activate all 20,000 genes, it is not possible...genes that, when turned on, make melanoma cells resistant to treatment with a BRAF inhibitor. (The test ... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ... -
CRISPRainbow and Genome Visualization
TypeBlog Post...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...for your audience. I ask myself that question constantly (and so should you!) Amount of information and... too little)? The answer is: it depends! The important point here is to pay attention to exactly what... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship...