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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 982 results for: IGH@

Showing: 921 - 940 of 982 results

  3. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

  8. Colony Formation Titering Assay

    Type
    Protocol
    ...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

  9. Pipetting Protocol

    Type
    Protocol
    ...to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving past... P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes the pipette...P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to...

  10. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    ... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...
Showing: 921 - 940 of 982 results