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We narrowed to 1,083 results for: IND

Showing: 921 - 940 of 1083 results

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... Sybr Green is a fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an...variance in your variant options, you’re likely to find an application that matches your needs. Happy quantifying...

  3. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...wavelengths (See this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...the University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas...the University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...-expression by using a plasmid with multiple, individual expression cassettes, having the genes expressed... the ability to spark translation initiation independent of the 5’ cap.  IRES elements are very useful...

  8. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...2015). Total Virus and Bacteria Concentrations in Indoor and Outdoor Air. Environmental Science & Technology...RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein. The EMBO Journal...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...insert-specific and back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages...

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...collaborators have completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all ...

  11. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...information or clarification on these vectors. Read on to find the answers to our most frequently asked lentiviral... designed specifically for transient expression (find some useful backbones here) or from viral transduction...

  13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...Don’t worry, we’ve got you covered! Here you will find some useful tips to help you make your plasmid construction...and a Level 2 multigene vector with two other independently regulated transcription units. Created with ...

  14. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...(Link opens in a new window) or isolating individual cells with cloning cylinders. Workflow Timeline This...when scanning the plate for individual cells, which can be difficult to find. Leave the cells undisturbed...medium (see below for more details) Day 1: Seed individual cells in a 96-well plate Day 2–14: Monitor cells...Some cell lines tend not to grow well in sparse/individual cultures due to lack of secreted growth factors...can be used to enhance the growth of cells in individual cultures. Other methods for enhancing single ...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...trypsinization and break up any cell clumps into individual cells by passing several times through a serological...

  15. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...column at the indentation in the tip. Equilibrate the column with 10 mL of RT Protein A/G binding buffer. re...kDa MWCO column, Millipore Sigma UFC903024 50 mL LoBind tubes, VWR 76289-498 Before Starting Wipe down ...bleach prior to use. Warm the affinity column, binding buffer, and elution buffer to room temperature ... 7.0 Autoclave or filter sterilize Protein A/G binding buffer (20 mM sodium phosphate buffer, pH 7.0) ...protease inhibitor cocktail. Add 1 part Protein A/G binding buffer to 1 part tissue culture supernatant and...column. Pro-Tip Gravitrap columns have a finite binding capacity. If your sample exceeds the capacity, ...multiple columns. For information about the total binding capacity for your columns, refer to the manufacturer...

  16. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...chart, such as the (Link opens in a new window) double digest finder tool from NEB . In a 1.5mL tube combine...Link opens in a new window) NEB's compatible cohesive ends chart . If you cannot find compatible sticky ...restriction enzymes, visit (Link opens in a new window) NEB . Restriction enzyme digestion is commonly...amount of enzyme for your reaction. However, keep in mind that restriction enzyme activity is determined under...purification kit, such as a (Link opens in a new window) QIAGEN DNA cleanup kit . See the enzyme manufacturer's...lose their function. If you are having difficulty finding an enzyme that cuts your vector's multiple cloning...certain restriction sites. See (Link opens in a new window) NEB's table of methylation sensitive restriction...

  17. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...Hybridization Sequence: The region of the primer that binds to the sequence to be amplified (usually 18-21bp... we want to amplify and design primers that will bind to and replicate it. The following image shows the...5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and we will add the EcoRI restriction site...end of a linear piece (see (Link opens in a new window) NEB for more information). Thus, we recommend ...internet search will lead you to (Link opens in a new window) here and many others). If we put the sequence ...reaction using a kit, such as the (Link opens in a new window) QIAquick PCR Purification Kit . The PCR product...anticipated size and that your band is strong (indicating that the PCR reaction worked and that you have...

  18. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    .... You can find even more video content on the Addgene Video Page . We hope that you'll find these protocols...cells Antibody Validation Using the Indirect ELISA Method Run an indirect ELISA against a purified antigen... Addgene Protocols On this page you'll find practical lab protocols that you can use for a wide... the lab. Name Description (Link opens in a new window) Link to Video Personal Protective Equipment (PPE...procedures. Name Description (Link opens in a new window) Link to Video Making LB Agar Plates Create plates...plasmids. Name Description (Link opens in a new window) Link to Video Restriction Cloning Assemble plasmids... DNA fragments in a single reaction Ligation Independent Cloning (LIC) Scarless cloning with Type II restriction...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ...HiFi (Link opens in a new window) and Gibson Assembly (Link opens in a new window) . They also sell ET SSB... new window) . NEB has other resources, such as a primer design tool (Link opens in a new window) . Gibson...Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB) to the isothermal reaction mix... New England Biolabs (NEB) (Link opens in a new window) sells DNA Assembly kits and master mixes, including...doi.org/10.1038/nmeth.1318 (Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, ...doi.org/10.1038/nmeth.1515 (Link opens in a new window) Rabe BA, Cepko C. (2020). A Simple Enhancement.../10.1101/2020.06.14.150979 (Link opens in a new window)...

  20. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...ultracentrifugation. The arrow indicates the 60–40% interface. The vertical black line indicates the location of the... 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle for...available under the name OptiPrep (Link opens in a new window) from Sigma-Aldrich. Protocol Video Watch the protocol...therapy 6.6 (1999): 973-985 (Link opens in a new window) . Figure 2: Left panel: Iodixanol gradient after...adeno-associated viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-... doi:10.1089/hgtb.2015.051 (Link opens in a new window) . Last reviewed on: November 7, 2023...
Showing: 921 - 940 of 1083 results