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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,012 results for: RING

Showing: 921 - 940 of 1012 results

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

  6. CRISPR Guide

    Type
    Guide
    ...scientists have modified for a wide range of genome engineering techniques . Collectively, these techniques are...R epeat) technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) ...CRISPR rapidly became the most popular genome engineering approach. Engineered CRISPR systems contain two...first and come back to HDR . NHEJ is a naturally occurring repair pathway and is the primary repair mechanism... for DSBs. While this pathway is efficient in repairing DSBs, it frequently causes small nucleotide insertions... the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve editing more ...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    ...FLAG) are cleaved by specific proteases without requiring the addition of an independent cleavage recognition...

  14. Modular Cloning Guide

    Type
    Guide
    ...Genome Engineering, CRISPR, Bacterial Expression Anke Becker Plasmids to perform genome engineering in Vibrio...applications—like building synthetic genetic circuits, engineering metabolic pathways, assembling gene-editing tools...MoClo Plant Parts III: Transformation & Genome Engineering Kit Plant Expression, CRISPR Daniel Voytas 93...framework for plant synthetic biology and multigene engineering, including CRISPR/Cas9 components. MoChlo: Modular...functional genomics, and more complex metabolic engineering and synthetic biology, compatible with PhytoBrick...to extend the MoClo-YTK (Dueber) for genetic engineering of S. cerevisiae , enabling larger multi-gene...Michael Sauer 62 plasmids for advanced strain engineering in P. pastoris (a.k.a. K. phaffii ), including...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...
Showing: 921 - 940 of 1012 results