We narrowed to 951 results for: cha

1. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   ... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

2. High Complexity Golden Gate Assembly with NEB’s DAD

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   Blog Post

   ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

3. Viral Vectors 101: Virus Safety

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   Blog Post

   ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just in case ...

4. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi, editing)   Figure...

5. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

6. Corralling and Cornering the Goldilocks Antibody

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   Blog Post

   ...application (different experiments/conditions can change binding ability – i.e., the antibody can no longer...

7. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   Blog Post

   ... Michael G. Lemieux...

8. Engaging with science and society at pgEd

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   Blog Post

   ...information briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream...

9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...No conference would be complete without CRISPR Michael Terns from of the University of Georgia studies...

10. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ... genetic redundancy (meaning, there’s more of a chance of seeing a phenotype since additional genes that...

12. Plasmids 101: Degron Tags

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    Blog Post

    ...CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of control. Protein degradation can be induced...

13. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    ...in house using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an...

14. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...The backbone modifications listed can refer to changes made to the original backbone by the depositing...

15. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...model.  If your expression system is not well characterized in terms of CRISPR use, you will want to invest...

16. Genome-wide Screening Using CRISPR

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    Blog Post

    ...this type of experiment can shed light on the mechanism by which cells gain resistance to drugs and can...

17. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...enjoy seeing how our quality control process has changed with the introduction of next generation sequencing...

18. An Introduction to Adenovirus

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    Blog Post

    ... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...domains of a protein that undergoes conformational change. Typically, two fluorescent proteins with overlapping...

20. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...with insertions or deletions (indels) and then characterizes the sequence and abundance of each particular...