We narrowed to 1,010 results for: ple

1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   ...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

   ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...

3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   ...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

   Type

   Blog Post

   ...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

5. Bringing Sustainable Practices to the Lab: Recycling

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   Blog Post

   ...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

6. Some Like it Hot: Thermostable GeoCas9

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   Blog Post

   ...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

7. CASTing Off for New Shores in Human Genome Editing

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   Blog Post

   ...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

8. Hot Plasmids - October 2022

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   Blog Post

   ...  Multiple Authors...

9. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

   ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

10. Meet the New Addgene Board Members!

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    Blog Post

    ...Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in...

11. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...

12. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    ...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

13. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    Blog Post

    ...contribution to the community because of..." This first couple of sentences - even if written in a clumsy language...

14. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post

    ...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

15. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    Blog Post

    ...synthetic vs. natural gene are essential and complementary tools in a time where biological engineering...

16. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced...

17. Antibodies 101: Epitope Tags

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    Blog Post

    ... 8 amino acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein...

18. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...might make it difficult to use NgAgo to disrupt multiple targets. Are you using NgAgo? Let us know how ...

19. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ... validated the serotonin sensor with proof-of-principle experiments that replicate current knowledge, ...

20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post

    ...multicolor reporters that will enable imaging of multiple proteases at once (Zhang et al., 2019). Studying...