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...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...

... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson...

...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...

...address a diverse set of biological inquiries. References Ogura, Teru, and Sota Hiraga. "Mini-F plasmid... Addgene.org Check out our molecular biology reference Search for a plasmid here Find plasmids from our...

...FRET experiments. You may have to design several different constructs to find one that could be used for ...Fluorescent protein FRET: the good, the bad and the ugly. Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann...

...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

...labelling techniques. These newer tools are also currently available at Addgene.  BioID2: A smaller improved...protein or RNA motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity...

...Over 25 million people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be...for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski...

...Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example, in a two-guide...Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice...

...Bioluminescence assay kits are available from different biotech companies which are enzyme-based mycoplasma...and a Bachelor's degree in Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous...

...Not sure where to start? Need a quick reference? Addgene's eBooks are here to help!...Collection Not sure where to start? Need a quick reference? Addgene's eBooks are here to help! 0 of 0 Selected...

...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...

...guide. Print out the guide and use it for quick reference when you're designing your next virus experiment...

...viral vectors and their elements; resources on different types of vectors, capsids, and tools; and helpful...

...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please check...

...101 eBook is designed to be a helpful desktop reference. Each chapter brings together all of our 101 blog...

...priority given to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent...

...Twitter feed will be all about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start...

...CRISPR, Addgene gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd...