1,192 results

Read about how mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is necessary for transfected cells.

Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

CRISPR-Cas9 technology appears in numerous publications, including its use for genome editing in monkeys and x-ray crystallography of Cas9's 3-D shape.

Tips for making the most of your mentoring relationship, including the ebook "Mentoring for Scientists".

Interview with Ed Boyden discussing his newest optogenetics tools and the questions that motivate neuroengineering.

This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

The advantages of participating in a peer mentoring group for scientists and how to form your own.

Tips for naming your plasmid in a way that is clear and informative to you and your colleagues.

Five steps to help graduate students and postdocs write their first grant proposal.

The third post in the mentoring series, this post describes ways you can approach finding a science mentor and advice on how to “make the ask.”

Learn about the importance of antibiotic resistance genes in plasmids. How they work, how to use them, and some great tips.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.

Identify which extracurricular activities will help you gain the skills you need to succeed in your science career.

Advice for choosing an advisor for a PhD or postdoctoral lab. Second in a series on Mentoring for Scientists.

An inside look at the ten most frequently distributed plasmid technologies at Addgene from the past ten years.

Three useful tips to help you organize your lab notebooks, keep track of tasks, and share protocols with other lab members.

Ten noteworthy developments in open science and scientific sharing of 2013.