...time. Both are helping researchers explore the complexity of translational control in cellular physiology... processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor...color switching, TRICK requires the following components: a GFP protein that binds to the coding region... with mRNAs usually translated in a specific compartment. Despite these limitations, these techniques ...

...strand in Figure 1 below) was free to anneal to complementary ssDNA molecules in vitro. Figure 1: Cas9...discovered that single stranded donor DNA: Complementary to the released strand, 127bp in length, With...target DNA that was accessible to anneal with complementary single stranded DNA. We thus wondered if such... any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know the mechanism...

...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...evolved into the Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful...

...because of the nature of the chemical reaction compared to using a high-intensity laser to rapidly excite...not required to initiate the reaction. Table 1 compares some general advantages and disadvantages of luminescence... Depending on the specific luciferase used, a compatible substrate must be provided for the enzyme to ... will result in luciferase expression and more complex cell treatments can be designed to further explore...

...guide for the quest of a perfect "fit". How competitive is the lab? Some labs have only one author plus...labs around the world. You may prefer a more competitive style to spur you to succeed or you might enjoy...enjoy working closely with others to accomplish your goals. You must understand your own style before making...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

...being depicted, we can’t forget that those other components are still there in real life.  Certainly referring...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...PIs, articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...

...Konermann et al. Plasmids here How do Cas13d enzymes compare to other Cas13s? Like other Cas13 enzymes, the ...cleavage pattern is necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small... the clear winner with median knockdown of 96% compared to 65% for shRNA, 53% for CRISPRi, and 66-80% ...Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively...

...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications. Challenges...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...

...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...membranes, organelles, and other subcellular compartments is via proteomics--the study of the distribution...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....

...available. Plasmids are continually added as they complete QC and you can find all available plasmids listed...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...batches of samples. As soon as that process is complete, QC scientists prepare Sanger reactions by mixing...are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first ~50 plasmids...