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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 471 - 480 of 1010 results

  1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-... Mary Gearing...

  2. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Griesbeck is motivated by a particular interest in monitoring the activity and biochemistry of living neurons...allowed the researchers to see tonic action potential firing in neurons and high resolution functional tracking...while in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple...cells at much lower concentrations than calcium, requiring greater sensitivity. He and his colleagues came...

  3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...innovations have improved the odds of mismatch repair favoring the edited strand. The first iteration of prime...introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch repair Even in PE3 and PE3b, prime editing...of the unedited strand Favor the edited strand during mismatch repair   ✓   ✓ ~ ~ ~ ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...recombinases and prime editing. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary feature... traits. The “biotech yeast” You’re probably wondering if the “biotech yeast” is S. pombe or S. cerevisiae...small, membrane-bound organelles essential for sequestering certain enzymes and performing specific oxidative...

  5. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock...sequencing. Maintain representation of the library during transformation First, use enough DNA and competent... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...making sure few/no plasmids have dropped out, and comparing pre- and post-amplification sequencing results...

  6. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ... review, and more lab research could be used to bring scientists and the public together to build consensus... it is followed. He’s PCSK9 paper, though not featuring a clinical pregnancy, was rejected by at least...Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on Addgene.org Read our...CRISPR plasmids by function Check out our genome engineering guide ...

  7. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Old Dominion University, who is majoring in marine biology and minoring in studio arts photography.  References...specific procedural instructions, including timing, preparing the smear, and mixing the stains and solutions.... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...

  8. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...limitations: they were not optimal for experiments requiring high signal to noise ratios, imaging of high frequency...Create yellow (v82) and lime green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the...and localization through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay...researchers introduce iGluSnFR3 in two additional anchoring domains – GPI and SGZ. Fig: 4 Sequence...

  9. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...organization, mentoring group, or department. Even if it doesn’t work out, it’s flattering to ask.  Show...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...There is a person on LinkedIn I once helped by answering one simple question so I accepted the connection...

  10. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles...ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs, the ITRs define the limit ... supplied by the production cell line (HEK-293) during production. Fun fact: There are 57 human adenovirus...
Showing: 471 - 480 of 1010 results