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We narrowed to 960 results for: comp

Showing: 471 - 480 of 960 results
  1. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ... to complete a six month work experience called a co-op to receive a degree. Northeastern compiles a list...Thankfully, my managers set aside time for me to complete an online social media certification on social...petting dogs, and winning the office Just Dance competition.    Special thanks to my manager, Sr. Marketing...
  2. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...projects spanning from the identification of plant compounds and insecticidal proteins to the development of... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...
  3. Strengthening Foundations: Reflections on 2025 and What's Ahead for 2026

    Type
    Blog Post
    ... constant evolution of scientific tools and computational approaches, we ensured that access to reliable... industry, as we make our collections more comprehensively accessible to scientific entrepreneurs. From...focus even more intently on efficiency without compromising the quality that defines Addgene. Many of these...Ranjith-Kumar, C. T., Guchhait, P., & Surjit, M. (2025). Comparison of immunogenicity and protection efficacy of ...
  4. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...CRISPR screen data are also available via comprehensive pre-compiled downloads and via a full-featured REST... to stably archived large-scale datasets by computational biologists. Figure 1: The BioGRID Open... from humans and other species. By accurately compiling genome-wide datasets, which are often obscured...
  5. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...synthetize all compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to...an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical...yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: Cells that have...
  6. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows for the labeling...PhiC31 irreversibly recombines one of the two competing attP sites to drive expression of either a stop...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...
  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...some extra guidance to understand the specific components that make up a plasmid and why each is important...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...used in research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid...
  8. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...donor with 40 bp homology arms. The HDR efficiency compared favorably to using WT Cas9 with the left gRNA ...
  9. An Addgene Summer Internship

    Type
    Blog Post
    ... had to get used to the workspaces, as well as complete the lab safety and cybersecurity training before...project is a major project given to each intern to complete and present on.    Figure 1: Addgene's...size should be a max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog...Toj-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They plan ...
  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...t guarantee a publication. There is an art to composing manuscripts and the ASM Scientific Writing and...writer, I was unsure if I should possibly take a composition class in the English Department. He suggested... coming to the writing workshop in D.C. had to compose and submit a manuscript that they were working ...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...
Showing: 471 - 480 of 960 results