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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 481 - 490 of 1010 results

  1. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major incentive...insoluble granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones...editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins...synthetic biology experiment Read our Genome Engineering Guide ...

  2. Antibodies 101: Isotypes

    Type
    Blog Post
    ...immune response. There are three main pathogen clearing functions or effector functions of the constant...each of the isotypes. IgM This isotype is produced during a primary immune response because all B cells begin...found in tissues. IgG is the predominant isotype during a secondary immune response, which is when the ...constant domains. This switching helps B cells adapt during the course of an immune response since it also ...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years to...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized...

  4. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...no photobleaching) but provides flexibility by pairing the luciferase with a variety of fluorescent proteins...light is only produced if a binding interaction brings the two luciferase components together. A similar...characteristic light. However, if a protein interaction brings the fluorescent protein close enough, energy is...

  5. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles...ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs, the ITRs define the limit ... supplied by the production cell line (HEK-293) during production. Fun fact: There are 57 human adenovirus...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...expanded for multiplexed imaging for advanced monitoring of different factors, in different or the same...stimuli and as well as changes in cellular dynamics during so-called housekeeping activities.     ...cell (sub)types for expression and downstream monitoring. Nervous about making a virus for your biosensor...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed...initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to know that...then ligates compatible DNA ends together, thus repairing the break. This is a simplified, streamlined ... ligation step determines how ends are resolved during nonhomologous end joining. Nat Commun 5: . https...

  8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” Before considering the use of pooled...that they could be used by people with little bioengineering expertise? “This is the next step in high-throughput...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with...

  9. Deep Dive: qPCR

    Type
    Blog Post
    ...to double-stranded, and finally, drop once more during a final temperature increase. If there is a single...should only be one peak, as everything should be denaturing or annealing at the same temperatures. If there.... Flipping your experimental and control values during analysis can allow you to see this fold change ... vol. 1160, DOI 10.1007/978-1-4939-0733-5_3, © Springer Science+Business Media New York 2014Bustin S; ... as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process. Sci Rep 8, 8716 (2018...

  10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural... prosthetic group PCB, PhyB and PIF6 dimerize, bringing each terminus of Cre together to restore Cre activity...to red light induces PhyBΔ and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer...as well as in different stages of development, ensuring the breadth and versatility of this tool. Find...
Showing: 481 - 490 of 1010 results