We narrowed to 1,019 results for: CAL;
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TypeBlog Post... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...
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CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...information is organized in wiki format and includes biochemical properties, previously established uses, and ... -
How Addgene Creates Content
TypeBlog Post... useful information on scientific literature, technical skills, and experiences from thousands of laboratories... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...of an open-access recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...from the Neuroscience Information Framework (NIF) called the Resource Identification Initiative. During ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...With our protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene... -
Video Library
TypeProtocol...culture Aseptic Technique Aseptic technique is a critical skill for success in numerous fields such as microbiology... AAV Titration by qPCR Use qPCR to measure the physical titer of your adeno-associated virus preparation... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... targeting the Fc region of an antibody, the so-called ‘constant’ region which is, quite usefully, both... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post... the desired level of antibodies is reached - typically within 2-4 months. After the animal has the desired... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...dosage in E. coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...too long in the incubator. The small colonies are called satellite colonies and they form when all the antibiotic... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... types of proteins. They started with another biological workhorse, Cre recombinase, which induces recombination... -
Promoters
TypeGuide...100-1000 base pairs long and are adjacent and typically upstream (5’) of the sense or coding strand of...transcribed by the RNA polymerase. DNA sequences called response elements are located within promoter regions... sequence thought to be functionally equivalent called the Pribnow box which usually consists of the six...Promoter The final portion of the promoter region is called the distal promoter which is upstream of the proximal...back on itself which helps to explain how many physically distant DNA sequences can affect transcription... -35 position, simply titled the -35 element, typically consists of the sequence TTGACA and this element... operator. Operons are common in prokayotes, specifically bacteria, but have also been discovered in eukaryotes... -
Immunocytochemistry
TypeProtocol...mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes Before Starting Refer to the manufacturer's... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...s outer membrane and allows the components to physically enter the cells so they can go to work. ... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...and Change in Undergraduate Biology Education: A Call to Action, Washington, DC. 2. President's Council... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...-humanized (Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 ...