We narrowed to 995 results for: IGH@;

...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...platform. Pour off the water in the sink. Take a brightfield image of the gel with an appropriate imaging ...25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There should ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...through a 0.2 µm PES filter. Aliquot and freeze upright at -20 °C Procedure Section 1: Affinity chromatography...and gently pour off the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs to the top...

...for a lay readership, and working with middle and high school students. One of these organizations was ...

...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ...

...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing...

...education. If you suspect that science communication might be for you, don’t wait. Start communicating now....

...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ...

...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...

... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it...

...Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated...

...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...

...some classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: ...

...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...

...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...

...with one or more of these parameters can lead to a high number of false negatives in Y2H screens. Testing...

...bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number...

...hosts. Also, self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009...

...amplification in the host cell. This enables relatively high and long-term expression of the reprogramming factors...

...externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists can make...