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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 974 results for: LAT

Showing: 961 - 974 of 974 results

  1. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    .... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

  2. Pipetting Protocol

    Type
    Protocol
    ...be in the liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...

  3. Modular Cloning Guide

    Type
    Guide
    ...Daniel Voytas 93 additional plasmids including regulatory elements, coding sequences, CRISPR parts GreenGate... for assembling large DNA fragments based on methylation-switching. 4G-Cloning Plasmid Kit Bacterial Expression...Expression, Mammalian Expression Stephan Gruber Platform for rapid assembly of expression vectors for multi-subunit...TALE-TFs) for genome editing and transcription modulation applications Golden Gate TALEN and TAL Effector...TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome Engineering, TALEN Takashi...Yamamoto Plasmids for construction of highly-active Platinum TALENs for use in a wide variety of target organisms...

  5. Guide to Using Pooled Libraries

    Type
    Guide
    ...name, pooled libraries are supplied as a mixed population of plasmids in a single tube. Pooled libraries...may lead to false positives or false negatives in later experiments, and can also negatively affect data...every plasmid is adequately represented in the population, you’ll need to infect many more cells than the...the cells die, removing their plasmids from the population, and only a small fraction survive. After the... serves as a non-selected control. These two populations are then sequenced using NGS to determine which...

  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...(FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell line. You...best practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR in cis...

  7. Cloning

    Type
    Guide
    ... molecular cloning scientists can amplify and manipulate genes of interest and then insert them into plasmids...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...

  8. Educational Resources

    Type
    Guide
    ...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

  9. Science Guides

    Type
    Guide
    ...start learning about various scientific concepts related to our plasmid collection. Find links to the guides...optics and genetic engineering to measure and manipulate cells (frequently neurons) and their governing...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ...balance. This will help you remove it from the scale later on as otherwise, the paper could stick to the surface...

  11. Ligation Independent Cloning

    Type
    Protocol
    ...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

  14. Addgene's eBook Collection

    Type
    Guide
    ...you need to know about plasmids, from parts, nomenclature, and uses to common promoters and cloning techniques...
Showing: 961 - 974 of 974 results