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Showing: 41 - 50 of 246 results

  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...dopamine D2/D3 receptors. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J...fluorescence-based ligand-binding assay. Scientific Reports, 6. https://doi.org/10.1038/srep25769 Grimm, J. B., & Lavis...Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038/s41592-021-01338-6 Grimm, J. B... vivo imaging. Nature Methods, 14(10), 987–994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y... partner for live cell imaging. PLoS ONE, 9(9). https://doi.org/10.1371/journal.pone.0106271 Heinrichs...fluorescent dyes. Nature Cell Biology, 11(S1), S7–S7. https://doi.org/10.1038/ncb1939 Liu, X., Yang, L., Long...mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-017-0037-8 Lucidi, M., ...

  2. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc.273.14.8447  Jain, A., & Srivastava...plasmids. FEMS Microbiology Letters, 348(2), 87–96. https://doi.org/10.1111/1574-6968.12241  Keen, N. T., ...in Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119(88)90117-5  Lale, R....Molecular Biology (Clifton, N.J.), 765, 327–343. https://doi.org/10.1007/978-1-61779-197-0_19  Meyer, R...host-range IncQ plasmids. Plasmid, 62(2), 57–70. https://doi.org/10.1016/j.plasmid.2009.05.001  Meyer, ...Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org/10.1007/BF00268809  Prior, J. E., Lynch...Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit.22695  Schmidhauser, T. J....

  3. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...RNA-targeting CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126/science.aaf5573 Broughton JP, ... detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen JS, Ma ...-stranded DNase activity. Science 360:436–439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS,... with CRISPR-Cas13a/C2c2. Science 356:438–442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS,... Cas13, Cas12a, and Csm6. Science 360:439–444 . https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije...diagnostics using CRISPR-Cas13. Science 360:444–448 . https://doi.org/10.1126/science.aas8836 Niewoehner O, ...CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh...

  4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...and applications. Gene Therapy, 17(2), 150–157. https://doi.org/10.1038/gt.2009.135. PubMed PMID: 19847206...Expression. Viruses: Essential Agents of Life, 147–175. https://doi.org/10.1007/978-94-007-4899-6_7. PubMed Central...human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt.2623. PubMed PMID: 23792628...Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/JCI35798. PubMed PMID: 18688286...infection. Journal of Virology, 70(2), 721–728. https://doi.org/10.1128/JVI.70.2.721-728.1996. PubMed ...genes and local hotspots. Cell, 110(4), 521–529. https://doi.org/10.1016/s0092-8674(02)00864-4. PubMed ...American Society of Gene Therapy, 17(8), 1316–1332. https://doi.org/10.1038/mt.2009.122. PubMed PMID: 19491821...

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... editing. Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587-021-01133-w Anzalone, A...breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J.,...editing outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Choi, J., Chen... editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025-z Doman, J. L...efficient prime editors. Cell, 186(18), 3983-4002.e26. https://doi.org/10.1016/j.cell.2023.07.039 Lin, Q., Jin...in plants. Nature Biotechnology, 39(8), 923–927. https://doi.org/10.1038/s41587-021-00868-w Nelson, J. ...efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Pandey, S.,...

  6. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...(red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For...these resources.   Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods...Protocols. American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-...Positive vs Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles...) a000414 (2010). Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612...gram-stain Hardy, J. Gram’s Serendipitous Stain. https://hardydiagnostics.com/wp-content/uploads/2016/05...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203...Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203...Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org/10.1038/s41587-023-01756-1. PMID: 37095348...derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic...brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038/s41565-023-01419-x. PMID: 37430038...in primates. Nature Communications, 14(1), 3345. https://doi.org/10.1038/s41467-023-38582-7. PMID: 37291094...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Gruntman, A. M., ...trials. Human Gene Therapy Methods, 26(2), 71–76. https://doi.org/10.1089/hgtb.2015.040 Initiative, F. B...Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G.,...and Clinical Development, 18(September), 98–118. https://doi.org/10.1016/j.omtm.2020.05.018 Tedersoo, L...scientific disciplines. Scientific Data, 8(1), 1–11. https://doi.org/10.1038/s41597-021-00981-0 Wright, J. ...formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez...

  9. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...transposon-encoded CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo...RNA-guided DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova...transposons. Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond... Bacteria Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha...CRISPR-associated transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda ...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... (e.g. http://openwetware.org/wiki/Protocols/Template). Nature Protocol Exchange Website: http://www.nature.com...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...started on our deposit page. Protocols.io Website: http://protocols.io/ Protocols.io is the creation of ... to use the resource. OpenWetWare Wikis Website: http://www.openwetware.org/wiki/Protocols OpenWetWare.org... and biomedical topics. Protocol Online Website: http://www.protocol-online.org/ Protocol Online is one... find useful for your research. LabGuru Website: http://www.labguru.com/ Labguru is a good place to go...such as Nature Protcols and NEB. Quartzy Website: https://www.quartzy.com/ Quartzy is a free web-based lab...
Showing: 41 - 50 of 246 results