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Showing: 41 - 50 of 1011 results
  1. Mentoring for Scientists: I Have a Mentor, What Now?

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    Blog Post
    ...about her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...want more great advice on mentoring, click below to download the "Mentoring for Scientists" ebook, which... the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series and additional... If you have been following the posts in this Mentoring for Scientists series, you have: realized the ...adding formality and active goal setting to your mentoring relationships, so that you can reap rewards in...Joanne's Reddit AMA Add a little formality to mentoring Just getting together and chatting with another...about their career path is a great start to a mentoring relationship, but if you don’t take this past ...
  2. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post
    ...brain at a level of abstraction that enables the engineering of its function." Once scientists can do that...big questions that motivate his interest in neuroengineering, and some of the optogenetic tools that are...are still missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can...set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other... neuroengineering...
  3. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post
    ...article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring questions I set out to answer...programs A formal mentoring program can be a great way to get started with mentoring. Read this Catalyst...Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. If your organization...also a great place to practice your mentoring skills. Online mentoring can work if both parties are diligent...my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking...School researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We should all ...organization has a mentoring program, consider tapping into it and helping make it work by being an educated...
  4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ... genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa...and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase complex... two transposase proteins (TnsB and C). While requiring fewer components for RNA-guided DNA integration...).  CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT.... For transposons to be specifically recognized during transposition, genetic cargos must be flanked by...currently studies CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter...high-efficiency, multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587...
  5. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids and ...are able... Thanks to everyone for being awesome during a tough time. We're all going to get through this...This is a time when we need as much open science sharing as we can get. The effects of Addgene’s brief...Addgene Team because they’re all doing amazing work during this difficult time. One function that rose to ...had to figure out a way to maintain our services during the crisis and maintain our workforce for when ...enough funds to make most of our payroll for the spring and early summer months. We are also extremely ...for grant-driven research being done in all labs sharing materials via Addgene. Perhaps if each of many ...
  6. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...without looking at what they’re delivering and where they’re delivering their payload to.   Figure...anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources....prioritization happens for good reasons. We began offering our viral vector service because we realized that... are available quickly. Beyond the service This brings us to the final piece of the viral vectors service...our AAV Data Hub as a community platform for the sharing of that exact data. Here, requestors can upload...their experimental data in a standardized format, sharing how well a given viral vector worked — or didn’...save time in selecting experimental conditions. Sharing our experiences Addgene’s plasmid repository gave...
  7. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post
    ...roles. Therefore, institutions are responsible for ensuring that either all affected scientists are paid above...here) pushing for exemptions for postdocs, and lowering of the proposed salary level for exemption. Discussions... are institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st...of 340 institutions with postdocs in science, engineering and health from the NSF’s 2014 Graduate Student...University of Delaware has a minimum of $36,360, requiring an increase of 30.6%. We have attempted to check...
  8. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...removal helps prevent the invading genome from “recovering” its original sequence. If you’re looking to ... High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional Resources on Addgene.org Check... Mary Gearing...
  9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ...base pairing, it has promise to be host-independent, beyond the oft published metabolic engineering realm...summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with...of research has shifted in recent years toward tinkering with regulatory networks and multiplex gene control... ‘biofactories’, most successes in metabolic engineering have been limited to the overexpression of various...scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward design....scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated the potential...violacein, a distinctive pigmented protein. By altering the transcriptional programs, the authors sequentially...
Showing: 41 - 50 of 1011 results