We narrowed to 911 results for: TRO

...Nicola Patron is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like...that go on between plants and their pathogens. As Patron explains it, her work has always been focused on...Modular Cloning (MoClo) Kit. What does it include? Patron: Sainsbury has a mandate to share and educate and...Addgene: Will the MoClo tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses...tips for those looking to get started with MoClo? Patron: The parts will be familiar to most plant scientists...there other tools you are working on for plants? Patron: Our lab and others at the Sainsbury are working... of engaging about science in that way for you? Patron: I started my Twitter account recently inspired...

...question. You can see that we have the beginning of control around the central dogma. We have the beginnings...priority; we need the keyboards and displays for control and visualization. Addgene: What do you envision...

...talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell...generation sequencing results to perform quality control on deposited plasmids. We’ve also talked about ...interface. We regularly use Snapgene for our quality control process because of its expansive feature library... main ways we use SnapGene for plasmid quality control are: To identify important plasmid features ...Identifying plasmid features While our quality control process for incoming plasmids can vary based on... quality control... plasmid quality control...

...pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G...plasmids. This is because in 2024 certain export control exceptions were made by the US Bureau of Industry...Industry and Security, pursuant to the international controls prescribed by the Australia Group, for these plasmids...Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging...researchers in countries with applicable export control exceptions for rabies and VSV-G genes, plasmids...strive to keep our information updated, export controls and regulations are complex and may change suddenly...

...and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017 for all incoming plasmids was a big...Addgene - we had used Sanger sequencing for quality control for over ten years. Now, thanks to a generous donation...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...The first step we take when performing quality control of newly deposited plasmids is to align the NGS... by the depositing lab, we call these “quality control (QC) issues.” We then ask the depositing laboratory...

... just label cells. If GFP could be co-opted to control gene expression, he could then selectively manipulate...promoters. Moving to an in vivo system, Tang et al. electroporated T-DDOGs, GFP, and a reporter construct into...TdTomato, whose expression was absent without electroporated GFP. They then tested T-DDOGs with two mouse...robust and specific. When tested in retinal electroporation studies, DsRed reporter expression was induced...

... lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting with two search terms...What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source organism for a gene...

...Their system is based on the AraC protein that controls transcription from the PBAD promoter. “AraC is... promoter. While none of the constructs gave as strong a signal as with the wild-type AraC, it still reached... of interest, under the pBAD promoter, can be controlled with light without the need of any cloning. ...

...an appropriate control? Many CRISPR screens use non-targeting guides as negative controls. Non-targeting...are well characterized and strategies exist to control for these faults. However, it’s still unclear if...screens and how best to design these screens to controls for flaws. Recently the Bassik Lab at Stanford...site in the genome. This means that while they control for many of the effects of gRNA and Cas9 expression...detecting Cas9 cutting. Safe targeting guides better control for non-specific toxicity than non-targeting guides... a coding region. Targeting these sites should control for the effects of guide expression and dsDNA breaks... the genome, and are commonly used as negative controls for genome-wide CRISPR screens; and 3) safe-targeting...