We narrowed to 345 results for: Ada
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TypeBlog Post...studied in 40 labs as a great model for plant adaptation and speciation. Their phenotypes are diverse ...captivity. Sticklebacks demonstrate unusual parallel adaptation to disparate environments so can be used study...
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Supporting Reproducibility with a Connected ELN
TypeBlog Post...support entry of required metadata, with the option of adding additional metadata in the repository after... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...Selection, prep, and testing of AAV enhancers in mice. Adapted from the Allen Institute's Genetic Tools Atlas....Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw... -
Hot Plasmids: Fall 2024
TypeBlog Post...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....design and studies of protein ubiquitination and degradation. Find the CRBNmidi plasmid here! Kroupova... -
Hot Plasmids - November 2023
TypeBlog Post...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0... -
Bioinformatics at Addgene
TypeBlog Post...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source... -
Transferable Skills Guide: Creativity
TypeBlog Post...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...such that prior formats are no longer supported/readable. Is the author or their host institution expected...Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...twist since it relies on the sequential nicking, degradation, and PCR-generation of a mutant version of the...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill, S. I., Parsley, K. L., Staal, F.... -
Hot Plasmids: Summer 2024
TypeBlog Post...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license... -
Hot Plasmids: Spring 2025
TypeBlog Post...Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0...displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...straight-forward, concise explanation of how to adapt our engineered devices, recombinase-based state ...not expressed in each state. To help researchers adapt GRSMs for their research, we provide MATLAB-based... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may want to declare...doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel... -
Finding Your Perfect Job After University
TypeBlog Post... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...functions more similarly to CRISPR/Cas9 systems adapted for eukaryotes in that Cas9-mediated cleavage induces...addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit the activity... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post... the balance of biogenesis and mitochondrial degradation in vitro and in vivo. Merged green and red ...long life-cycle of a protein from expression to degradation. Chemical approaches may require expensive peptides... -
New Coomassie Protocol Video
TypeBlog Post...Antibodies." Hot tip! This protocol can easily be adapted for other types of proteins. If this video ... -
CRISPR 101 eBook...en español
TypeBlog Post...está disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos... -
The Codon Guide to Synthetic Biology
TypeBlog Post...whenever possible. Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry Schaefer, NCI, NIH ... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...Flow Compensation and Flow Cytometry Controls Priyamvada Prathima, who wrote a helpful guide to understanding... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...to any experiment and can help prevent future headaches. To help you plan PEI transfections for AAV packaging... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog Post...and leveraging your unique skills set while also adapting to needs as they arise. Tips on applying for industry... -
Celebrating One Million Plasmids Shared!
TypeBlog Post...21, 2018 As Addgene grows, we have been able to adapt to the needs of the scientific community. For example... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog Post... assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for work in ... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...multiple genes, and more. The researchers now plan to adapt the new tool for use with a broader array of Cas9s... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...differences in the kynurenine pathway of tryptophan degradation. It turns out that activation of the PGC-1a1 ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post... power lies in the clever ways scientists have adapted them for use in the laboratory. The discovery ... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog Post...top career blog posts into one comprehensive, downloadable guide: the Addgene Science Career Guide. We ... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...protein family and they generate color! They've been adapted for many molecular biology methods such as allelic... -
Let There Be LITE Plasmids
TypeBlog Post...dynamic organ,” Konermann explained. “It helps us adapt to influences in our environments. We are able to... -
Editor's Choice, August 2016
TypeBlog Post...Developing Lab Management Software for Biology Adapting Toehold Switches to Detect Zika Virus As always... -
Science as Art
TypeBlog Post...front pages of newspapers. Science is now being adapted to inspire to a new generation. Daniel Stoupin... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...pharmaceutical companies, academic labs have been slower to adapt, likely due to the necessary time and resource investment... -
What's New in CRISPR - March 2020
TypeBlog Post...ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. Caulobacter crescentus... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...activation/repression. dCas9 has also previously been adapted to fluorescently label a sequence matching its ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...the mechanism of dual-RNA-guided DNA cleavage in adaptive bacterial immunity and its potential applications...