We narrowed to 550 results for: nol

...their hot-off-the-presses papers, the awesome new technology they’re utilizing, and the tricks they’ve used...plasmids in your own lab. Summaries of the latest technologies and breakthrough research. Insights about ...a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing and the...

...the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This video goes over... over AAV vector purification using an iodixanol column gradient. We'll show the loading and unloading...instructions. AAV titration by qPCR using SYBR green technology Here’s some advice on how to successfully set...

... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

...; Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer...abundances using SYBR Green I and real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui...Reproducible Data the First Time, Trends in Biotechnology, Volume 37, Issue 7, 2019, Pages 761-774, SSN... for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 – 133Tichopad A; Dilger...

... the United States, and many universities and Technology Transfer Offices were, at least, temporarily ...                           California Institute of Technology             Johns Hopkins University                           ...will initiate a conversation with you and your technology transfer office to discuss the possibilities ...

...2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene....it is requested from Addgene many times as the technology disseminates throughout the research community...

...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...

... to Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389...adverse reactions to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug....2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz...

...They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB...activation in bacteria Article contributed by Will Arnold Baojun Wang’s lab recently developed a CRISPR tool...

...tools by making their own fluorescent protein technologies widely available to the research community. ... a key advantage over other inducible enzyme technologies. She hopes that other researchers will be able... HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first case and...

... via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https://doi.org/10.3389/fimmu.2021.730825... Gradinaru V, Fox AS. (2023). Advances in AAV technology for delivering genetically encoded cargo to the...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...widespread gene transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt...tropism of viral vectors in tissues. Nature Biotechnology. https://doi.org/10.1038/s41587-022-01648-w ...

...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

...γ-retrovirus packaging cell line developed by Garry Nolan at Stanford (Swift, 2001) contains Gag-Pol and either...resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient...cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: 10.1002/...

... and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, ... the TurboID plasmids here! Branon et al. Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...

...CRISPR 101" post. Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive...introduce digital PCR as an emerging validation technology. Digital PCR has several advantages over mismatch...CRISPR-Cas nucleases in human cells. Nature Biotechnology 31, 822–826 (2013). PubMed PMID: 23792628. PubMed...

...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

...Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others. Each method is unique...biologists have harnessed it to evolve cloning technology. CcdB: A potent toxin... The ccdB gene, located...thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially a ...

...to your cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first...Voytas groups described a new Golden Gate-based technology for genome editing which allowed for the ordered...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting...

... the limitations of current state of the art technologies. Therefore, take time to review the literature...insight into what the desirable features of future technologies should offer in order to build upon and address...proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary data...

...President's Council of Advisors on Science and Technology [PCAST] (2012). Engage to Excel: Producing One...Additional College Graduates with Degrees in Science, Technology, Engineering and Mathematics, Washington, DC:...DC: US Government Office of Science and Technology. 3. Duina, A. A., Miller, M.E., & Keeney, J.B. Budding...

...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field... obstacles for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges...taken advantage of what our current delivery technologies are suited for. For example, lipid nanoparticle...

...targeting of long non-coding RNA genes. Nature biotechnology. 2020;38(5):573. doi:10.1038/s41587-020-0428... cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038...Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant...

...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the...antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing to turn...antibodies. Since recombinant antibodies are a newer technology, it is important to show through qualitative ...

...variety of research sectors to provide open source technologies and spaces where interested parties can do research...is a passion for learning and having fun with technology, so feel free to contact your local space and...Biocurious.org, Derek is bringing cutting edge technologies to Victoria, BC. Follow Derek on Twitter @derekja...

...Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology...discovery through the creation of innovative technologies. To date his group has develop several popular...catalytically active Cas9 nuclease." Nature biotechnology (2015). PubMed PMID: 26436575. PubMed Central...

...indel formation (gene disruption) with this new technology. To dig more into what researchers felt about...genome editing with NgAgo, this does not mean the technology is dead in the water. On the bright side, successful...Natronobacterium gregoryi Argonaute." Nature biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn...

...T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...the production of recombinant Erythropoietin. J Biotechnol. 2020 Feb 10;309:44-52. doi: 10.1016/j.jbiotec...beads from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002...

...metabolic cassettes (Zhang et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet...CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter @Vopinator...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang...

...approximately 20,000 droplets using water-oil emulsion technology. Each droplet contains the ingredients for amplification... added benefit, replicates are built into the technology. One well (containing thousands of droplets) ...titration, there are many applications for the technology.  Droplet digital PCR is well suited for the ...

... CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID: 27918548. ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids...CRISPR-Cas Cpf1 nucleases in human cells." Nat Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids...

...showed APEX2 can use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne group for click... tapes for intracellular recordings. Nature Biotechnology, 41(5), 631–639. doi: 10.1038/s41587-022-01524...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife...

...editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587...with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587...and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/...

... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin...at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa...

...) either produced de novo using yeast display technology or produced from hybridoma antibodies developed...synthetic rAbs, developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...science, IPI hosts events and training courses on technologies and laboratory techniques related to antibody...

...Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018). Shcherbakova, D.M. et al...encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377 (2021). Shcherbakova, D.M. Near-infrared... fluorescent protein for in vivo imaging. Nat Biotechnol 29, 757-761 (2011).   ...

...David Liu’s lab by combining Cas9 DNA-targeting technology with a fused cytidine deaminase to create C→T...activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587...Cas domain compatibility and activity. Nature Biotechnology, 38(7), 883–891. https://doi.org/10.1038/s41587...

...avidin-biotin technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 ...interaction with avidin for molecular biology and biotechnology purposes.  Once a polypeptide is biotinylated...

...impact (15). Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This... 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern research,...Trump’s first budget Environmental Science and Technology: Crossing The Imaginary Line The New York Times...