We narrowed to 402 results for: MUT

1. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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   ... focusing on safety and the ability to generate mutant microbial strains faster than ever before. 29:54...

2. New Viral Vectors - Summer 2024

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   ...viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV9 Biosensors (Control) Li New viral service...

3. What's New in CRISPR - May 2019

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   .... Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of...

4. What's New in CRISPR - Spring 2019

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   ...DNA strand. The lab then found gain-of-function mutations that allows it to be repurposed for double strand...

5. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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   ...infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral sequences...

6. Fluorescent Proteins 101: History of Fluorescent Proteins

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   ... protein’s structure and also discovered many mutations that alter and improve its function and shift ...

7. What's New in CRISPR - December 2019

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   ...Single base editor that induces C-to-T and A-to-G mutations Nozomu Yachie’s lab recently developed a base ...

8. Modulate the Activity of 17 Signaling Pathways with One Kit!

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   ...pathways, they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes...

9. Six Spooky Science Stories and Halloween at Addgene

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   ...discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective...

10. New Norepinephrine Indicators: nLightG and nLightR

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    ...adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and nLightR uses...

11. What's New in CRISPR - September 2019

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    ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

12. Working 9-5: an Addgene Co-op

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    ...useful to me as some days it was not as easy to commute as others. I know some fear that remote work would...

13. 6 Steps to Submitting a Resume That Gets Seen

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    ...manager on paper or as an email send directly from a mutual connection. If the organization is small, any person...

14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    ... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to...

15. Hot Plasmids and Viral Preps - July 2021

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    ...control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia...

16. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    ...confirm the identity of the gene and check for any mutations. Once we identify the gene, we can easily annotate...

17. Hot Plasmids - October 2022

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    ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

18. Plasmids 101: Gene, enhancer, and promoter trapping

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    ...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...

19. Build Your CRISPR Vocabulary

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    ...other hand, viruses can evolve to evade CRISPR by mutating these sequences. Pro tip! Various labs have created...

20. Sleeping Beauty Awakens for Genome Engineering

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    ... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

21. History of CRISPR Cas - A tale of survival and evolution

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    ...CRISPR/Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases...

22. New Viral Vectors - Winter 2025

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    ...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...

23. Hot Plasmids and Viral Preps - September 2021

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    ...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

24. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    ...cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains...

25. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...Kwart, including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing...

26. In Vivo Biotinylation of Bacterial Fusion Proteins

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    ...receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin molecules. The in ...

27. Antibodies 101: Epitope Tags

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    ...Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of the same protein...

28. 10 Great Guest Posts We're Thankful For

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    ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...

29. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

30. Plasmid Cloning by PCR

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    ...to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes more important...

31. Networking Follow-up: I Met a Person. Now What?

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    ...can’t really help you. The exception is having a mutual acquaintance connect you to someone who you need...

32. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

33. DAP arrays for multiplex gene editing

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    ...any CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...

34. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    ...later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase...

35. Science Career Options

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    ...will you stay in one location? How long is the commute? Will the job require public speaking? Will you...

36. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    ...if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in...

37. Components of CRISPR/Cas9

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    ...Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs (see table below...

38. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    ...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...

39. Viral Vectors 101: Biosensors

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    ...and are ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics...

40. GCE4All: Making Genetic Code Expansion Accessible

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    ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

41. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ... contain the expected features and are free of mutation.  Once the samples arrive, each group of plates...

42. Viral Vectors 101: Viral Applications

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    ... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...

43. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...immune measures and correlating them with tumor mutations and clinical responses. This platform has the ...

44. Luminescent Imaging with Nano-lanterns

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    ...described the first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent...

45. Top Requested Lentivirus and AAV of 2016

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    ...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

46. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...

47. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This...

48. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

49. Antibodies 101: Affinity Reagents

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    ...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ...

50. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach to select ...