...discussions about the pros and cons of different fabrics while we worked. We also had discussions about the difference...grad student. I chose to focus on misinformation about sexual and reproductive health, as the stigma around... misinformation can flourish. I’ve never forgot about all the anxious, whispered questions from friends...the study design or by their preconceived ideas about the controversial medical issues. Participants ...none of the participants had preconceived opinions about “adeolitis.” The participants in my study were ...make reusable cloth menstrual pads while we talked about the environmental impact of disposable products....contexts that shape what people know and believe about the world. It is vital to understand what people...

...and Jeffrey Tabor have been working on making E. coli light responsive since 2005, when Tabor was working...these classic systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in...for the computational problem of edge detection. Tabor et al. subsequently engineered a second photosensitive... and highest level of “output” signal, was only about 10-fold, precluding the study of gene expression...-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi...Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, ...Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya...

... advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists connect...saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had used ...science communication skills, while also learning about new scientific developments and helping scientists... found this paper. I wrote an Addgene blog post about it and shared the paper with my lab since one of...project. I was invited to participate on a panel about “Social Media for Science Communication” at the ...Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do not capture...social media to consistently engage with others about your topic(s) of interest. Hopefully through these...

...Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...transferable skills guide: Problem-Solving. I don’t know about your experience in the lab, but mine was plagued...the job description, but this skill is not just about reacting to technical issues. This skill also comes...I try to clarify what people mean when they ask about “problem solving skills” and look at how scientists...effectively resolves the issue.  If this sounds like about seven different skills to you, you are not wrong...Imagine your problem is just a question you have about the world. You might do some background research...went through your head as you made observations about the issue, assessed potential protocol modifications...

...Northeastern University, she interviewed Addgenies about their career path. Graduate school: Do I or ...or don’t I? I asked the nine Addgenies about why they did or did not go to grad school. QC Scientist Alyssa...our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think that my...Perkins noted that there is a more emphasis today about non-academia and industry jobs. Since there are ...to keep my values in mind as I applied. Thinking about this made me curious. I wondered what my coworkers...view of science. It should be really open and collaborative.” She knew she wanted to leave academia, and...from my co-op. It has been so insightful to learn about different career paths, successes and failures, ...

...School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies...s never too early to start networking! Learning about careers at Addgene Afterwards, the students split... was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And several of our visitors...visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome...awesome!”. The students also got to learn about intellectual property from our Tech Transfer team members...are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy said...work to help improve K-12 education? Let us know about your educational events in the comments section ...

...carrying a synthetic gene drive were to escape the laboratory, the drive could eventually spread through the...mistake would necessarily extend far beyond the laboratory and seriously damage public trust in scientists... can spread through populations of transgenic laboratory organisms but not wild organisms. For example...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...Reproductive confinement involves working with laboratory organisms that can't reproduce with wild ones...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety...population and immunizes it against the first drive. Laboratories interested in building candidate gene drives...

...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach...outreach - if you'd like to learn more about Science communication check out these SciComm blog posts.... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

...scientists to make genomic alterations, bringing about a revolution in genome engineering, with new techniques...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane, allowing...as lipid-mediated transfection (B in the figure above), are in the early stages of development. David ...proteins harboring receptor ligands (C in the figure above), which result in the internalization of Cas9-gRNA...show promise for in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...

...didn’t have to worry about having to hunt down sources later on.  A comment about writing: initially I...exists. Once you pick a topic, try to be specific about exactly what aspect of the field you plan to review...themes in the field and to learn what people cared about. I also used reviews to get a list of research papers...highlighted a few dozen things that I didn’t know about. Instead of reading a paper and getting my bearings...them. No really, just start writing! Don’t worry about grammar or formatting or continuity. Also don’t ...worry if you feel like you don’t still know enough about the topic. Just get as many words down on the page...between office hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews or...

...The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in... of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an Full Professor in Biochemistry with... CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in plants.     ...Rodrigo Arturo Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...

... will also serve as a good mentor. To read more about what makes a good mentor, see the previous post ... and personnel management?  If you are thinking about a non-academic path, will the PI support this path...Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor...time with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members... past lab members. Most scientists love to talk about their past lab experiences and will be willing to...members, neighbors or alumni give you information about the PI that concerns you, don’t just brush this ...Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting...

...Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow ...crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system...by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic...are introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream...downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be...an introduction to live cell imaging Learn more about the origin of the Brainbow system Visit our fluorescent... Addgene’s fluorescent protein collection Learn about fluorescent tools for in vivo imaging ...

...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate...Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone...samples. The goal is to not only learn something about the microbial diversity in different soil conditions...community lab in a city near you. To learn more about what we’re up to or how you can help BosLab, visit...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

...communication in remote collaborations Transferable Skills Guide: External collaborations Transferable Skills... stint may be ideal for you. Here, we will chat about who might want to explore this role, how to seek...scientist is distinct from equipment sharing or a collaboration – it requires re-location of the scientist to...reasons Visiting gives you access to possible new collaborations and fresh perspectives. For trainee level researchers...future work placements. The chance to learn and collaborate all while experiencing a new city or maybe even...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach out and ask if...

... communication, and in particular communication about experimental methods and approaches. It’s very easy...everything will be fine. It won’t. When we hear about issues with reproducibility, time and time again...the problem comes down to inadequate information about what the original researchers actually did. Admittedly...Communication comes in many forms—from transparency about data to allow others to see what you actually found...fits together to make for a transparent and collaborative research environment. Journals as enforcers ...of methods sections, including clear information about reagents, and why other publishers and organizations... in reproducibility jail and more effective collaboration to drive the progress of science. Sounds like...

...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they... in the Microbiology Community Sign Up to Write About Stem Cells Resources on the Addgene Wesbite Find...

...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...guide A quick guide to all of the tabs listed above and their functions. We hope that these updates...

...designed (listed above) worked well and I got clear bands using the conditions above. 2. Design your primers...essential for experiments but that nobody talks about. After months of troubleshooting, those people who...details. The entire process from start to finish took about 1 month. Cas9 delivery Overview: Before any ...is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9...original publication they see GFP expression in about 20% of their Cas9-expressing cells. So, even when... gene has a weird mutation that you didn’t know about (which could affect the ability of the gRNA to bind...engineering has come. For comparison, in 2007 it took about 3 months to knockout a single allele in a human ...

...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...been using yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...communicating science to the public. You can find out more about Clare at the Biology Dept. site.  References 1. ...PMC4596657. 5. Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ...

...the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining .... For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the end of ... and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... Plasmids, plasmids everywhere To find out more about gene edited hiPS cell lines generated using the ...line. The editing strategy and design described above can be used to create similar donor plasmids for...microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines generated using these...

...introduction to my area, a line about the research I will be doing, and a line about what I hope to discover....themselves many times and will have useful advice about what reviewers in your area are looking for. Remember...One way to add originality to your work is to collaborate with specialists in different fields that can...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...outcomes can include poster presentations, talks about your work, or even the successful use of your preliminary...quality application. Even following this advice, only about 50% of my grant applications have been successful...on your grant application to your peers and collaborators can also help you get some critical insight ...

...more about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across... connecting us with him. Learn more about our recent collaboration with Seeding Labs here. Special thanks...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...Addgene Podcast.     Additional Resources Learn about Seeding Lab's Work with Lilongwe Univeristy of Agriculture...How You Can Contribute to the Addgene Blog Learn about Addgene Plasmid Donations for Rare Disease Research...

...Blog Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing Learn about Homology Directed...editing specificity. We’re excited to learn more about xCas9 - here’s what we know so far! Why do we ...point mutations, also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM...in mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When...are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9 is going to be flat...Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History ...

...negotiate with friends, partners, and/or children about everything from what you’re going to eat for dinner...Negotiation as a zero sum game Some people think about negotiation as a zero sum game - that is, if I get... three examples depend on many specific details about the individuals, each example provides a scenario... difficult because of the need to be thoughtful about what is most important to each person. It can especially...of us associate with negotiating (check out more about conflict resolution). How to develop negotiation...before, during, or after a negotiation to think about the situation, your reaction, and the outcome. How... able to take my vacation? I want to learn more about a different area of the company. How can I negotiate...

...record readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular... for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research...thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings in the...changes quickly and harmlessly. Learn much more about the laws and their possible repercussions in Gary...This post leads readers to our latest podcast all about graduate student life and how establishing a thriving...to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope you all... labor laws...

...wonderful tangents. Some groups asked about vaccines, others were curious about the agricultural repercussions...roles and responsibilities, I have to think a lot about how to balance those roles. I’m a father of three...community. This past year, I spent some time thinking about how I could combine all of these roles by reaching...guest presentations. If you’re a scientist thinking about reaching out to your community’s schools, here are...accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a single evening...DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular mechanism, I was careful to keep... I gave my presentation five times to groups of about 50 students each, and each group went on its own...

...think about. Choosing a topic and gauging interest The first thing to do if you’ve thought about starting... discussions. Beyond the excitement of learning about new cutting edge science, researchers of all varieties...help stay current on new and emerging work by collaboratively discussing recent publications in a specific...up. At Addgene, we wanted to think more closely about the wealth of high-throughput sequencing (HTS) data...the paper.  Before the first journal club, think about the meeting itself A journal club should aim to ...published science. They also serve as a way to learn about work outside of one's own field in a structured ...hope that with some of the information described above you feel empowered to start your own journal club...

...created The Addictive Brain to spread awareness about how scientists utilize tax-payer money and government...Here, Sadangi and his team answer a few questions about The Addictive Brain and science communication for...science communication? At The Addictive Brain, we collaborate with scientists from around the world and help...numerous feedbacks from our non-scientist readers about how our articles have helped them understand different...at The Addictive Brain? This year, we plan to collaborate more with physicists, chemists, and mathematicians...having to extensively use Google search. Be careful about hidden connotations behind a word. Non-scientists...Sign up to receive our science career guide Learn about different careers in science communication Resources...

...for gene manipulation together with information about the genome are powerful resources for investigating...zebrafish genome was published in 2013, revealing that about 70% of human genes have a counterpart in zebrafish...networks that regulate processes from development to metabolism to fertility. Several zebrafish-specific gene...gene expression databases that provide information about when and where genes are active in developing embryos...genome sequences become available, new hypotheses about specific genetic interactions arise and tools to...genome, their long three-month generation time and labor-intensive maintenance compared to invertebrate model...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...

...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What is Science in the News...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

...blog Read about COVID-19 research on the blog Visit our guide for external collaborations Resources ...scientific sharing. Already we’ve seen articles about COVID-19 on preprint servers and in journals, and...vary, everyone we spoke with sees sharing and collaboration as the best way that the scientific community...useful resources during this time. Facilitate collaborations within the scientific community The team behind... Canada is a database that provides information about reagents, funding and volunteer opportunities, protocols...CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data and experimental...

... passionate about. Check out my previous post if you're looking for more information about the benefits...every spurious result under the sun. Academia is about convincing results, and nothing is more convincing...is your passion? Even if no one else is excited about it, distinguish yourself with this passion. “Love...presentations and of course, networking and collaborating.” Getting out of the lab also “opens the student's...emphasizes the importance of passion, hard work, collaboration, and staying honest with personal goals. Stay...George Church for answering our burning questions about how they manage and motivate their labs! Update...

...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...screen shot and overview of the table can be found above). These categories are further discussed below (.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...

...allow you to collaborate within the platform and avoid countless emails. Important collaborative functionalities...of the software. We identified that when talking about ELN costs and limited budgets, “a large percentage...Besides that, academic institutions tend to think about the long term, so they need a solution for which...institution. In the study, respondents were also asked about how important ease of use is for them (if they are...or partners to access your data? If you are collaborating with other labs or clients, you might need share...comments, added files, notes etc. Detailed records about every action performed in the lab (user, date, time...Additional Resources on the Addgene Blog Learn about more tools for reproducibility 5 great apps for ...

...rely on what I know about those particular teams at that given time. I think about how previous iterations...disagreements. In fact, conflict resolution isn’t just about resolving conflicts between other people, but resolving...normally get along well with each other disagree about a specific decision, direction, or goal. Resolving...of lapsed communication. Making some assumptions about how something should be communicated is necessary...confusion around... Ownership: Misunderstandings about who is responsible for what tasks or projects is...conflicts.  Transitioning a conflict into a collaboration may not be the easiest resolution to accomplish...cooperation: avoiding, competing, accommodating, collaborating, and compromising. Though there may be a time...

...Science Communication blog series, you’ll learn about Caitlin Runne-Janczy, a Product Development Manager...G-proteins at the time. This program takes what you know about it [your area of research], and teaches you how ... hustle on the side. “I actually stumbled upon, about 6 months in, this opportunity to do scientific editing....” But Caitlin’s persistence paid off: “I spent about 6 months just editing and searching for a job and...Additional Resources on the Addgene Blog Learn more about alternative science careers here Be like Caitlin...just for good measure, here’s a few other posts about developing transferable skills while in grad school...school Resources on Addgene.org Learn more about careers at Addgene ...

...end up in academic positions (I’ve even blogged about this here and here); however, simply put, I moved...better and because I really like talking to people about and communicating science. The path to science communication...realized that I’m energized by talking to people about science and that I love explaining things to people...I spent a couple of days talking to researchers about their work, picking up plasmid deposits, and trying...glad I did a PhD and even more glad that I thought about careers outside of academia during my graduate training...to many different fields. If you start thinking about preparing yourself for a career while still in graduate...Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive...

... You can learn more about recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring ... resource for the neuroscience community called NABOR (Neuroscience AntiBody Open Resource). We are delighted... validating these tools. While the focus of the NABOR platform will be neuroscience reagents and data,...Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation and acquisition... the NIH’s BRAIN Initiative has been funding laboratories, such as Dr. Trimmer’s lab, to create recombinant...Check out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal antibodies ...

...manipulation more hands on While teaching courses about gene therapy and genetic manipulation, I and other...with some other professors, I went home thinking about how to solve this problem. I didn’t want to face...therapeutic strategies work. Find Addgene Blog posts about other educational tools Playing BUILD YOUR PLASMID...winner? Ultimately, society should be! This is a collaborative game, as it should be in real scientific practice...practice transferable skills like team building, collaborative critical thinking, and communication among others.... My impression was that they had clearer ideas about gene therapy in the following lessons. And let me...biology Learn how BioBuilder can help teach students about Synthetic Biology Find Arabidopsis educational resources...

...collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...deal more information that one might like to store about FPs than is currently represented at fpvis (complete...thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized, community-editable...resource for storage and dissemination of information about FPs. FPbase.org FPbase is a project written in ...comprehensive community resource for information about fluorescent proteins.  It was designed with the ... there is plenty of room for improvement and elaboration.  Current projects include incorporation of biosensors...protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store...

...each of your meeting types (see above). We’ve provided some tips above for what works well at Addgene,...opposite effect. A great meeting should produce collaboration - a sense of dialogue and community among participants...meeting to discuss something difficult, be excited about the prospect of working toward a solution. At the...sections for each person to add a bullet or two about what they are working on. Building this into the...purpose of the meeting (see “defining the scope” above) or if you can cut it down to thirty minutes. ...some tips. Before long, you will be running collaborative, informative, energizing meetings all your own...

...start their own academic research laboratory. How does one even go about starting a lab? Obviously, funding...always helps. But how does one build a strong collaborative research team motivated by similar visions and...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer: ... has to deal with. My inbox currently stands at about 65,000 messages and dealing with email can be half...the first couple of hires to build a strong, collaborative team. And take advantage of the many resources...Medical School) for taking the time to speak with us about adding new scientists to their labs. Further Reading...

...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...In early 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services....provides viral vector technical service (details about virus production protocols and quality control can...material. The Outreach Team is getting the word out about this new resource and providing fantastically useful...Outreach Team has been making sure scientists know about the new initiative and serving all the new educational...

...You never know when you will meet a potential collaborator Some of these people you meet will review your...know personally Scientists in your field will know about grants and projects that might help your work Tenure... your card, they have to give you theirs! Think about how you want to introduce yourself and what you ...those you would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting...questions you are pondering and be ready to talk about them. People love to help especially when solving...“Is this seat taken? Can I join you?” Next talk about the food and then move on to science. More great...conference meal etiquette are here. Don’t only talk about science and work – casual socializing is how people...

... related blog posts Learn about our cancer collection pages Read more about the tumor microenvironment...the tumor. How do microenvironment enzymes and metabolic reprograming influence tumors development? Being... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...particularly interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6...resources page  Find plasmids for your research Learn about Addgene's viral service ...

...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids...always emphasized the need for open science and collaboration. The field already has comprehensive reference...planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check out their...elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative polyadenylation...addition to the wealth of open resources described above, there were many plasmid tools presented at the ...by the worm community. For additional resources about C. elegans and the plasmids we have at Addgene check...

...and gives you a sense of what people are excited about. Following scientists in fields outside your main...research organizations, and universities often tweet about publications in the fields of research they support...journals: They publish papers, so naturally they tweet about papers too. You can follow journals related to your...field of research or larger journals that publish about a broad range of topics.  Twitter paper bots: Twitter...number of retweets, likes, and comments on a tweet about a paper can help you gauge how “hot” a paper is....papers that the scientific community is talking about and jumping into the discussion! Semantic Scholar...and search for papers later. Pro tip Learn more about authors by exploring author pages (for example, ...

...heard about it, I got excited from a computer scientist perspective. But, the more I thought about it, ...maintains a list of active spaces near you.   Learning about C. elegans with the OpenWorm Project One such space... an example of one of the problems we work collaboratively to solve, we want to focus our research on ...mutant known as CX51 that exhibits motor defects above 25 degrees. Computer scientists and biologists work... work together to compare the predictions about the effects this mutation has on locomotion in our computer...optimizing the health of domesticated cats and learning about feline microbiomes. One of the aspects of this project...