Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 535 results for: try;

Showing: 201 - 250 of 535 results

  8. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...cells Don’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most...Khilji recently acquired her Master’s degree in Biochemistry from Freie Universität Berlin, Germany. She ...

  11. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... notable exception If you have a sample you are trying to compensate in the top right corner of a flow...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...buttons. For anyone just starting to use flow cytometry, it’s generally recommended to start compensating... vs. Spectral Flow Cytometry...

  12. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Addgene’s repository. Other researchers, eager to try this new technology, were quick to request CRISPR...deposits and distribution by country. (A) Total CRISPR plasmids deposited by country; square size is proportional...requested by country; square size is proportional to the number of plasmids requested by each country. Countries...therefore looked at depositors and requestors by country, and graphed the top ten countries for each group...proportional to number of plasmids deposited by each country. Countries shown are top 10 depositing countries...

  17. Western Blot

    Type
    Protocol
    ...location of the protein of interest. You may need to try a variety of lysis buffers to find the best for your...Collection Antibody Blog Posts Antibody Guide Immunocytochemistry Protocol Recombinant Antibody Purification...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    ...organizations like Science from Scientists, which try to bring real scientists into schools to provide ... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...

  22. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...it was promising enough to keep exploring and to try developing a variant with an enhanced response. Evolution...researchers from a range of fields, like quantum chemistry and protein biophysics, as well as inventors and...effects relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447...

  24. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...and free for all non-profit uses. We invite you to try ICE today and let us know how it works for you! Read...the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth analysis will...

  25. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter of time... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with...Minnesota State (and now Professor of Chemistry and Biochemistry at the University of San Diego), decided...Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth...presented their findings at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of...inquiry and research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification...dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323...

  26. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...applying for technical support positions, you should try to match your expertise with that of the company....are often found in sales or lab-based roles in industry. What to look forward to in a technical support...offer an easy transition from an academic lab to industry without being too commercial or “sales-y”. The...be a good jumping off point for other roles in industry. Many thanks to Eric Perkins and Stephanie Hays...

  28. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...of saying “I have excellent communication skills” try giving an example, like “I have extensive communication... Should I write a cover letter? If you’re in a country where cover letters are common, the answer is usually...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover letters...if ever, sees cover letters submitted for their entry-level jobs and does not require or request them ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...positions. Many did not require cover letters for entry-level positions, as at that stage, cover letters...

  31. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...organisms, they can be problematic for researchers trying to express proteins in heterologous hosts. If you...

  33. Plasmids 101: Protein tags

    Type
    Blog Post
    ...their protein of interest. After several decades of trying to address these challenges, researchers have amassed... used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding... used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires...

  35. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...not always a straightforward process. Rather than try to directly detect your gene of interest, scientists...advantage of an element called an Internal Ribosome Entry Site (IRES) to allow for initiation of translation...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et...

  37. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters...E. coli tryptophan operon Repressible Gets turned off with high levels of cellular tryptophan. lac General...

  38. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...microfuge tube and vortex. Pro-Tip You may need to try a few different concentrations of antigen to determine...101: The Four ELISAs and When to Use Them Immunocytochemistry Protocol Recombinant Antibody Purification...

  41. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... Addgene. If you’ve given any of these systems a try, we’d love to hear your feedback!   Thank you to...Jieze Zhang is an undergraduate student studying biochemistry at the University of Southern California. He...obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese Academy of Sciences. He joined Shanghai... new generation of engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered...

  42. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...

  44. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet... quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4. Hindson CM, Chevillet...

  47. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ... new to the mARG expression are advised to first try to produce GVs in their own lab using the most reliable...is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar...Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209 (2017). Bourdeau, R. W. et al....

  49. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... know a lot about and that you care about. Don’t try to present topics that are unfamiliar to you because...Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...

  50. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...directed mutagenesis. In addition, you may want to try different concentrations of template (e.g. 0.1-1.0...in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID: 8470801....
Showing: 201 - 250 of 535 results